SCF Stem Cell Factor Rat Recombinant Protein

• Description:

  Stem cell factor Rat Recombinant produced in E. coli is a single, non-glycosylated polypeptide chain containing 164 amino acids (26-189) and having a molecular mass of 18.4 kDa. The Rat SCF is purified by proprietary chromatographic techniques.

• Synonyms:

  Kit ligand Precursor; C-kit ligand; SCF; Mast cell growth factor; MGF; SF; KL-1; Kitl; DKFZp686F2250; Hematopoietic growth factor KL

• Gene Name:

  KITLG

• UniProt:

  P21581

• Reactivity:

  Rat

• Source:

  Escherichia coli

• Sequence:

  [object Object]

• Purification:

  Greater than 98.0% as determined by (a) Analysis by SEC-HPLC and (b) Analysis by SDS-PAGE.

• Bioactivity:

  The ED50 is determined by the dose-dependant stimulation of the proliferation of human TF-1 cells which is less than 10 ng/ml, corresponding to a specific activity of 100,000units/mg.

• Form:

  Sterile Filtered White lyophilized (freeze-dried) powder.

• Reconstitution:

  It is recommended to reconstitute the lyophilized SCF in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

• Function:

  Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5- trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.

• Shipping Conditions:

  Available

• Storage Conditions:

  Lyophilized rat SCF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution SCF should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Please prevent freeze-thaw cycles.

• Other Gene Names:

  Kit ligand

• Subcellular Location:

  Isoform 1: Cell membrane