IL-1-beta Interleukin-1 beta Human Recombinant Protein, HEK

• Description:

  Interleukin-1 beta Human Recombinant produced in HEK cells is a glycosylated monomer, having a molecular weight range of 18-25kDa due to glycosylation. The IL-1 beta is purified by proprietary chromatographic techniques.

• Synonyms:

  Catabolin; Lymphocyte-activating factor (LAF) ; Endogenous Pyrogen (EP) ; Leukocyte Endogenous Mediator (LEM) ; Mononuclear Cell Factor (MCF) ; IL1F2; IL-1 beta

• Gene Name:

  IL1B

• UniProt:

  P01584

• Reactivity:

  Human

• Source:

  HEK

• Sequence:

  [object Object]

• Purification:

  Greater than 95% as obsereved by SDS-PAGE.

• Bioactivity:

  The specific activity was determined by the dose-dependent stimulation of the proliferation of mouse D10S cells and is typically 0.02-0.08ng/ml.

• Form:

  Sterile Filtered White lyophilized (freeze-dried) powder.

• Reconstitution:

  It is recommended to reconstitute the lyophilized IL-1b in sterile PBS containing 0.1% endotoxin-free recombinant HSA.

• Function:

  Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.

• Shipping Conditions:

  Available

• Storage Conditions:

  Lyophilized IL-1 beta although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL-1 beta should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) . Please prevent freeze-thaw cycles.

• Other Gene Names:

  Interleukin-1 beta

• Subcellular Location:

  Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.