Mouse CD99L2 ELISA Kit PicoKine®, Carrier-free

• Background:

  CD99 antigen-like protein 2 is a protein that in humans is encoded by the CD99L2 gene. It is mapped to X; X A7.3. This gene encodes a cell-surface protein that is similar to CD99. A similar protein in mouse functions as an adhesion molecule during leukocyte extravasation. Alternate splicing results in multiple transcript variants.

• :

  heparin, EDTA or citrate

• Description:

  Mouse CD99L2 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Cd99l2 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	467	427	448	472	453	17.72	3.9%
  Sample 2	1310	1396	1545	1357	1402	87.99	6.2%
  Sample 3	7832	7176	7216	8274	7624	456.32	5.9%

  *number of samples for each test n=16.

• Synonyms:

  CD99 antigen-like protein 2; MIC2-like protein 1; CD99; Cd99l2; Mic2l1

• Gene Name:

  CD99 antigen-like 2

• Gene ID:

  171486

• UniProt:

  Q8BIF0

• Reactivity:

  Mouse

• Cross Reactivity:

  There is no detectable cross-reactivity with other relevant proteins.

• Immunogen:

  Expression system for standard: E.coli; Immunogen sequence: D26-G161

• Tissue Specificity:

  Highly expressed in the nervous system, including brain, dentate nucleus of hippocampus, granular and Purkinje cells of cerebellum, brain stem nucleus and choroid plexus. Expressed in peripheral blood T- and B-cells and neutrophils. Almost undetectable in bone marrow-derived neutrophils. Also expressed in thymocytes with higher expression in cortical thymocytes than in medullary thymocytes. Expressed at high levels in testis (mostly in germ cells and Sertoli cells) and ovary (mostly in granulosa cells). Expressed in lung, heart, kidney and liver; however, expression in heart, kidney and liver seems restricted to endothelial cells. Highly expressed in endothelial cells and to a lower level in vascular smooth muscle cells. Low expression in spleen.

• Applications:

  ELISA

• Standard:

  20 ng/tube

• Sample Type:

  cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

• Detection Range:

  312 pg/ml - 20,000 pg/ml

• Sensitivity:

  <50 pg/ml

• Function:

  Plays a role in a late step of leukocyte extravasation helping cells to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Homophilic adhesion molecule, but these interactions may not be required for cell aggregation.

• Components:

  Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

• Storage Conditions:

  Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

• Specificity:

  Natural and recombinant mouse CD99L2

• Subcellular Location:

  Cell membrane. Single-pass type I membrane protein. Extracellular side. Cell junction.