Rat Contactin-1 ELISA Kit PicoKine®, Carrier-free

• Background:

  Contactin 1, also known as CNTN1, is a protein which in humans is encoded by the CNTN1 gene. It is mapped to 7q35. The protein encoded by this gene is a member of the immunoglobulin superfamily. It is a glycosylphosphatidylinositol (GPI)-anchored neuronal membrane protein that functions as a cell adhesion molecule. It may play a role in the formation of axon connections in the developing nervous system. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene.

• :

  heparin or EDTA

• Description:

  Rat Contactin-1 ELISA Kit PicoKine® (96 Tests). Quantitate Rat Cntn1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	217	222	227	247	228	11.38	4.9%
  Sample 2	1103	1131	1250	1268	1188	71.96	6.0%
  Sample 3	3628	4052	4148	3796	3906	205.70	5.2%

  *number of samples for each test n=16.

• Synonyms:

  Contactin-1; Neural cell surface protein F3; Cntn1

• Gene Name:

  contactin 1

• Gene ID:

  117258

• UniProt:

  Q63198

• Reactivity:

  Rat

• Cross Reactivity:

  There is no detectable cross-reactivity with other relevant proteins.

• Immunogen:

  Expression system for standard: NS0; Immunogen sequence: E21-S1001

• Tissue Specificity:

  Expressed by neurons, oligodendrocytes and their progenitors. Myelination regulates the expression being downregualted when neurons are in contact with Schwann cells.

• Applications:

  ELISA

• Standard:

  10 ng/tube

• Sample Type:

  cell culture supernatants, serum and plasma (heparin, EDTA).

• Detection Range:

  156 pg/ml - 10,000 pg/ml

• Sensitivity:

  <50 pg/ml

• Function:

  Contactins mediate cell surface interactions during nervous system development. Involved in the formation of paranodal axo-glial junctions in myelinated peripheral nerves and in the signaling between axons and myelinating glial cells via its association with CNTNAP1. Participates in oligodendrocytes generation by acting as a ligand of NOTCH1. Its association with NOTCH1 promotes NOTCH1 activation through the released notch intracellular domain (NICD) and subsequent translocation to the nucleus. Interaction with TNR induces a repulsion of neurons and an inhibition of neurite outgrowth.

• Components:

  Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

• Storage Conditions:

  Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

• Specificity:

  Natural and recombinant rat Contactin-1

• Subcellular Location:

  Cell membrane. GPI-anchor.