Human Matrilin-3 ELISA Kit PicoKine®, Carrier-free

• Background:

  Matrilin-3 is a protein that in humans is encoded by the MATN3 gene. This gene is mapped to 2p24.1. This gene encodes a member of von Willebrand factor A domain containing protein family. This family of proteins is thought to be involved in the formation of filamentous networks in the extracellular matrices of various tissues. This protein contains two von Willebrand factor A domains; it is present in the cartilage extracellular matrix and has a role in the development and homeostasis of cartilage and bone. Mutations in this gene result in multiple epiphyseal dysplasia.

• :

  heparin or EDTA

• Description:

  Human Matrilin-3 ELISA Kit PicoKine® (96 Tests). Quantitate Human MATN3 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	125	143	138	129	133	7.11	5.3%
  Sample 2	689	800	745	802	759	46.43	6.1%
  Sample 3	2209	2281	2219	2542	2312	135.20	5.8%

  *number of samples for each test n=16.

• Synonyms:

  Matrilin-3; MATN3

• Gene Name:

  matrilin 3

• Gene ID:

  4148

• UniProt:

  O15232

• Reactivity:

  Human

• Cross Reactivity:

  There is no detectable cross-reactivity with other relevant proteins.

• Immunogen:

  Expression system for standard: NS0; Immunogen sequence: D29-R486

• Tissue Specificity:

  Expressed only in cartilaginous tissues, such as vertebrae, ribs and shoulders.

• Applications:

  ELISA

• Standard:

  10 ng/tube

• Sample Type:

  cell culture supernatants, serum and plasma (heparin, EDTA).

• Detection Range:

  93.7 pg/ml - 6,000 pg/ml

• Sensitivity:

  <50 pg/ml

• Function:

  Major component of the extracellular matrix of cartilage and may play a role in the formation of extracellular filamentous networks.

• Components:

  Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

• Storage Conditions:

  Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

• Specificity:

  Natural and recombinant human Matrilin-3

• Subcellular Location:

  Secreted.

• Protein Name:

  Matrilin-3