Mouse IFNAR1 ELISA Kit PicoKine®, Carrier-free

• Background:

  Interferon-alpha/beta receptor alpha chain is a protein that in humans is encoded by the IFNAR1 gene. It is mapped to 16 C3.3; 16 52.98 cM. The protein encoded by this gene is a type I membrane protein that forms one of the two chains of a receptor for interferons alpha and beta. Binding and activation of the receptor stimulates Janus protein kinases, which in turn phosphorylate several proteins, including STAT1 and STAT2. The encoded protein also functions as an antiviral factor.

• :

  heparin or EDTA

• Description:

  Mouse IFNAR1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse IFNAR1 in cell culture supernatants, serum and plasma (heparin or EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	123	112	114	105	113	6.42	5.6%
  Sample 2	489	411	498	489	471	35.26	7.4%
  Sample 3	1822	1958	1775	1754	1827	79.40	4.3%

  *number of samples for each test n=16.

• Gene Name:

  interferon (alpha and beta) receptor 1

• Gene ID:

  15975

• UniProt:

  P33896

• Reactivity:

  Mouse

• Cross Reactivity:

  There is no detectable cross-reactivity with other relevant proteins.

• Immunogen:

  Expression system for standard: NS0; Immunogen sequence: E27-T429

• Applications:

  ELISA

• Standard:

  10 ng/tube

• Sample Type:

  cell culture supernatants, serum and plasma (heparin or EDTA)

• Detection Range:

  62.5 pg/ml - 4,000 pg/ml

• Sensitivity:

  <10 pg/ml

• Function:

  Component of the receptor for type I interferons, including interferons alpha, IFNB1 and IFNW1. Functions in general as heterodimer with IFNAR2. Type I interferon binding activates the JAK-STAT signaling cascade, and triggers tyrosine phosphorylation of a number of proteins including JAKs, TYK2, STAT proteins and the IFNR alpha- and beta-subunits themselves. Can form an active IFNB1 receptor by itself and activate a signaling cascade that does not involve activation of the JAK-STAT pathway. Contributes to modulate the innate immune response to bacterial lipopolysaccharide.

• Components:

  Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

• Storage Conditions:

  Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

• Specificity:

  Natural and recombinant mouse IFNAR1

• Subcellular Location:

  Cell membrane. Single-pass type I membrane protein. Late endosome. Lysosome.