Human TRKC ELISA Kit PicoKine®, Carrier-free

Human TRKC ELISA Kit PicoKine®, Carrier-free

• Background: Tropomyosin receptor kinase C? (TrkC),?also known as?NT-3 growth factor receptor,?neurotrophic tyrosine kinase receptor type 3, or?TrkC tyrosine kinase?is a?protein?that in humans is encoded by the?NTRK3?gene.This gene encodes a member of the neurotrophic tyrosine receptor kinase (NTRK) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signalling through this kinase leads to cell differentiation and may play a role in the development of proprioceptive neurons that sense body position. Mutations in this gene have been associated with medulloblastomas, secretory breast carcinomas and other cancers. Several transcript variants encoding different isoforms have been found for this gene.?
• Description: Human TRKC ELISA Kit PicoKine® (96 Tests). Quantitate Human NTRK3 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml . The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
• Synonyms: NT-3 growth factor receptor; GP145-TrkC; Trk-C; Neurotrophic tyrosine kinase receptor type 3; TrkC tyrosine kinase; NTRK3; TRKC
• Gene Name: neurotrophic receptor tyrosine kinase 3
• Gene ID: 4916
• UniProt: Q16288
• Reactivity: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: NS0; Immunogen sequence: C32-A428
• Tissue Specificity: Widely expressed but mainly in nervous tissue. Isoform 2 is expressed at higher levels in adult brain than in fetal brain.
• Applications: ELISA
• Standard: 10 ng/tube
• Sample Type: cell culture supernatants, serum and plasma (heparin, EDTA).
• Detection Range: 156 pg/ml - 10,000 pg/ml
• Sensitivity: <10 pg/ml
• Function: Receptor tyrosine kinase involved in nervous system and probably heart development. Upon binding of its ligand NTF3/neurotrophin-3, NTRK3 autophosphorylates and activates different signaling pathways, including the phosphatidylinositol 3-kinase/AKT and the MAPK pathways, that control cell survival and differentiation.
• Components: Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4
• Storage Conditions: Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
• Specificity: Natural and recombinant human TRKC
• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	330	342	297	342	327	18.41	5.6%
  Sample 2	1741	1582	1616	1731	1667	69.63	4.1%
  Sample 3	4941	4243	4806	4622	4653	262.39	5.6%

  *number of samples for each test n=16.
• : heparin or EDTA
• Subcellular Location: Membrane; Single-pass type I membrane protein.