Human PD-L2/B7-DC ELISA Kit PicoKine®, Carrier-free

Human PD-L2/B7-DC ELISA Kit PicoKine®, Carrier-free

• Background: Programmed cell death 1 ligand 2 (also known as PD-L2, B7-DC) is a protein that in humans is encoded by the PDCD1LG2 gene. It is mapped to chromosome 9p24.2. PD-L2 is one of two ligands for programmed death-1 (PD-1), a member of the CD28 family of immuno-receptors. Northern blot analysis detect wide expression of PDL2, with highest levels in placenta, heart, pancreas, lung, and liver, and lower levels in spleen, lymph nodes, and thymus. PDL2 negatively regulates T cells and plays an essential role in immune tolerance.
• Description: Human PD-L2/B7-DC ELISA Kit PicoKine® (96 Tests). Quantitate Human PDCD1LG2 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 12pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
• Synonyms: Programmed cell death 1 ligand 2; PD-1 ligand 2; PD-L2; PDCD1 ligand 2; Programmed death ligand 2; Butyrophilin B7-DC; B7-DC; CD273; PDCD1LG2; B7DC, CD273, PDCD1L2, PDL2;
• Gene Name: programmed cell death 1 ligand 2
• Gene ID: 80380
• UniProt: Q9BQ51
• Reactivity: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: NS0; Immunogen sequence: L20-T220
• Tissue Specificity: Highly expressed in heart, placenta, pancreas, lung and liver and weakly expressed in spleen, lymph nodes and thymus.
• Applications: ELISA
• Standard: 10 ng/tube
• Sample Type: cell culture supernatants, serum and plasma (heparin, EDTA).
• Detection Range: 62.5 pg/ml - 4,000 pg/ml
• Sensitivity: <12 pg/ml
• Function: Involved in the costimulatory signal, essential for T- cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production (By similarity). .
• Components: Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4
• Storage Conditions: Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
• Specificity: Natural and recombinant human PD-L2
• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	133	126	127	117	125	5.71	4.5%
  Sample 2	396	453	452	415	429	24.44	5.6%
  Sample 3	998	945	1041	1035	1004	38.22	3.8%

  *number of samples for each test n=16.
• : heparin or EDTA
• Subcellular Location: Isoform 3: Secreted