Human SAP/PTX2/APCS ELISA Kit PicoKine®, Carrier-free

• Background:

  Amyloid P component, serum (SAP), also known as PTX2 or APCS, is the identical serum form of amyloid P component (AP), a 25kDa pentameric protein first identified as the pentagonal constituent of in vivo pathological deposits called "amyloid". It belongs to the pentraxins family, characterised by calcium dependent ligand binding and distinctive flattened β-jellyroll structure similar to that of the legume lectins. This gene is mapped to 1q23.2. The binding of the encoded protein to proteins in the pathological amyloid cross-beta fold suggests its possible role as a chaperone. This protein is also thought to control the degradation of chromatin. It has been demonstrated that this protein binds to apoptotic cells at an early stage, which raises the possibility that it is involved in dealing with apoptotic cells in vivo.

• :

  heparin or EDTA

• Description:

  Human SAP/PTX2 ELISA Kit PicoKine® (96 Tests). Quantitate Human APCS in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	2959	2863	2697	2962	2870	107.66	3.7%
  Sample 2	13066	13637	15280	15579	14390	1063.69	7.3%
  Sample 3	40903	43039	47063	46975	44495	2634.74	5.9%

  *number of samples for each test n=16.

• Synonyms:

  Serum amyloid P-component;SAP;9.5S alpha-1-glycoprotein;Serum amyloid P-component (1-203);APCS;PTX2;

• Gene Name:

  Serum amyloid P-component

• Gene ID:

  325

• UniProt:

  P02743

• Reactivity:

  Human

• Cross Reactivity:

  There is no detectable cross-reactivity with other relevant proteins.

• Immunogen:

  Expression system for standard: E.coli; Immunogen sequence: H20-V223

• Tissue Specificity:

  Found in serum and urine. .

• Applications:

  ELISA

• Standard:

  100 ng/tube

• Sample Type:

  cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

• Detection Range:

  1.56 ng/ml - 100 ng/ml

• Sensitivity:

  <10 pg/ml

• Function:

  Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. May also function as a calcium-dependent lectin.

• Components:

  Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

• Storage Conditions:

  Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

• Calculated Molecular Weight:

  25387 MW

• Specificity:

  Natural and recombinant human SAP

• Subcellular Location:

  Secreted.

• Protein Name:

  Serum amyloid P-component