Mouse FCRN/FCGRT ELISA Kit PicoKine®, Carrier-free

Mouse FCRN/FCGRT ELISA Kit PicoKine®, Carrier-free

• Background: The neonatal Fc receptor (FcRn), also known as the Brambell receptor, is a protein that in humans is encoded by the FCGRT gene. The neonatal Fc receptor is an Fc receptor which is similar in structure to the MHC class I molecule and also associates with beta-2-microglobulin. It was first discovered in rodents as a unique receptor capable of transporting IgG from mother's milk across the epithelium of newborn rodent's gut into the newborn's bloodstream. Further studies revealed a similar receptor in humans, leading to the naming as a neonatal Fc receptor. In humans, however, it is found in the placenta to help facilitate transport of mother's IgG to the growing fetus and it has also been shown to play a role in monitoring IgG turnover. Neonatal Fc receptor expression is up-regulated by the proinflammatory cytokine,TNF-α, and down-regulated by IFN-γ.
• Description: Mouse FCRN/FCGRT ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Fcgrt in cell culture supernatants, serum, plasma (heparin, EDTA) and cell lysates. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
• Synonyms: IgG receptor FcRn large subunit p51;FcRn;IgG Fc fragment receptor transporter alpha chain;Neonatal Fc receptor;Fcgrt;Fcrn;
• Gene Name: IgG receptor FcRn large subunit p51
• Gene ID: 14132
• UniProt: Q61559
• Reactivity: Mouse
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: NS0; Immunogen sequence: S22-S297
• Tissue Specificity: Intestinal epithelium of suckling rodents. Expressed in neonatal intestine and fetal yolk sac.
• Applications: ELISA
• Standard: 10 ng/tube
• Sample Type: cell culture supernatants, serum, plasma (heparin, EDTA) and cell lysates.
• Detection Range: 93.7 pg/ml - 6,000 pg/ml
• Sensitivity: <10 pg/ml
• Function: Binds to the Fc region of monomeric immunoglobulins gamma. Mediates the selective uptake of IgG from milk and helps newborn animals to acquire passive immunity. IgG in the milk is bound at the apical surface of the intestinal epithelium. The resultant FcRn-IgG complexes are transcytosed across the intestinal epithelium and IgG is released from FcRn into blood or tissue fluids.
• Components: Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4
• Storage Conditions: Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
• Specificity: Natural and recombinant mouse FCRN
• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	132	123	130	122	126	4.32	3.4%
  Sample 2	1019	1070	915	953	989	59.65	6%
  Sample 3	2423	2361	2207	2520	2377	113.7	4.7%

  *number of samples for each test n=16.
• : heparin or EDTA
• Calculated Molecular Weight: 40093 MW
• Subcellular Location: Cell membrane; Single-pass type I membrane protein.
• Protein Name: IgG receptor FcRn large subunit p51