Human SPINT1/HAI-1 ELISA Kit PicoKine®, Carrier-free

• Background:

  Kunitz-type protease inhibitor 1 is an enzyme that in humans is encoded by the SPINT1 gene. It is a member of the Kunitz family of serine protease inhibitors. The SPINT1 gene encodes an inhibitor of hepatocyte growth factor activator (HGFAC) and matriptase and is involved in degradation of the extracellular matrix. SPINT1 is produced as an active membrane-associated protein and is proteolytically cleaved and secreted. Alternative splicing results in multiple variants encoding different isoforms.

• :

  heparin

• Description:

  Human SPINT1/HAI-1 ELISA Kit PicoKine® (96 Tests). Quantitate Human SPINT1 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	222	251	218	240	232	13.4	5.7%
  Sample 2	1803	1701	1702	1716	1730	42.27	2.4%
  Sample 3	3990	4390	4302	4668	4337	241.86	5.5%

  *number of samples for each test n=16.

• Synonyms:

  Kunitz-type protease inhibitor 1;Hepatocyte growth factor activator inhibitor type 1;HAI-1;SPINT1;HAI1;UNQ223/PRO256;

• Gene Name:

  Kunitz-type protease inhibitor 1

• Gene ID:

  6692

• UniProt:

  O43278

• Reactivity:

  Human

• Cross Reactivity:

  There is no detectable cross-reactivity with other relevant proteins.

• Immunogen:

  Expression system for standard: NS0; Immunogen sequence: G36-L529

• Applications:

  ELISA

• Standard:

  10 ng/tube

• Sample Type:

  cell culture supernatants, serum and plasma (heparin).

• Detection Range:

  156 pg/ml - 10,000 pg/ml

• Sensitivity:

  <15 pg/ml

• Function:

  Inhibitor of HGF activator. Also acts as an inhibitor of matriptase (ST14).

• Components:

  Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

• Storage Conditions:

  Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

• Calculated Molecular Weight:

  58398 MW

• Specificity:

  Natural and recombinant human SPINT1

• Subcellular Location:

  Secreted.

• Protein Name:

  Kunitz-type protease inhibitor 1