Mouse LDLR / OLR1 ELISA Kit PicoKine®, Carrier-free

• Background:

  The Low-Density Lipoprotein (LDL) Receptor is a mosaic protein of 839 amino acids (after removal of 21-amino acid signal peptide) that mediates the endocytosis of cholesterol-rich LDL. In humans, the LDL receptor protein is encoded by the LDLR gene. It belongs to the Low density lipoprotein receptor gene family. The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia. Alternate splicing results in multiple transcript variants.

• :

  heparin

• Description:

  Mouse LDLR / OLR1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Ldlr in cell culture supernatants, serum, plasma (heparin) and urine. Sensitivity: 12pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	138	141	137	131	136	3.63	2.6%
  Sample 2	599	522	564	529	553	30.71	5.5%
  Sample 3	1472	1333	1447	1397	1412	53.12	3.7%

  *number of samples for each test n=16.

• Synonyms:

  Low-density lipoprotein receptor;LDL receptor;Ldlr;

• Gene Name:

  Low-density lipoprotein receptor

• Gene ID:

  16835

• UniProt:

  P35951

• Reactivity:

  Mouse

• Cross Reactivity:

  There is no detectable cross-reactivity with other relevant proteins.

• Immunogen:

  Expression system for standard: NS0; Immunogen sequence: A22-R790

• Applications:

  ELISA

• Standard:

  10 ng/tube

• Sample Type:

  cell culture supernatants, serum, plasma (heparin) and urine.

• Detection Range:

  62.5 pg/ml - 4,000 pg/ml

• Sensitivity:

  <12 pg/ml

• Function:

  Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits.

• Components:

  Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

• Storage Conditions:

  Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

• Calculated Molecular Weight:

  94947 MW

• Specificity:

  Natural and recombinant mouse LDLR

• Subcellular Location:

  Cell membrane; Single-pass type I membrane protein. Endomembrane system; Single-pass type I membrane protein. Membrane, clathrin-coated pit ; Single-pass type I membrane protein . Golgi apparatus . Early endosome . Late endosome . Cell surface . Lysosome . Found distributed from the plasma membrane to intracellular compartments. Localizes to the Golgi apparatus, early and late endosomes/lysosomes and cell surface in the presence of PCSK9 (By similarity). .

• Protein Name:

  Low-density lipoprotein receptor