Human CD73/NT5E/Ecto-5'-nucleotidase ELISA Kit PicoKine®, Carrier-free

• Background:

  5'-nucleotidase (5'-NT), also known as ecto-5'-nucleotidase or CD73 (Cluster of Differentiation 73), is an enzyme that in humans is encoded by the NT5E gene. Ecto-5-prime-nucleotidase catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. And the enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. Rareallelic variants are associated with a syndrome of adult-onset calcification of joints and arteries (CALJA) affecting the iliac, femoral, and tibial arteries reducing circulation in the legs and the joints of the hands and feet causing pain.

• :

  heparin or EDTA

• Description:

  Human CD73/NT5E/Ecto-5'-nucleotidase ELISA Kit PicoKine® (96 Tests). Quantitate Human NT5E in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	230	235	243	260	242	11.37	4.6%
  Sample 2	1941	1870	1830	1891	1883	40.01	2.1%
  Sample 3	5361	4741	5455	5425	5245	293.24	5.5%

  *number of samples for each test n=16.

• Synonyms:

  5'-nucleotidase;5'-NT;3.1.3.5;Ecto-5'-nucleotidase;CD73;NT5E;NT5, NTE;

• Gene Name:

  NT5E

• Gene ID:

  4907

• UniProt:

  Q6NZX3

• Reactivity:

  Human

• Cross Reactivity:

  There is no detectable cross-reactivity with other relevant proteins.

• Immunogen:

  Expression system for standard: CHO; Immunogen sequence: W27-K547

• Applications:

  ELISA

• Standard:

  10 ng/tube

• Sample Type:

  cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

• Detection Range:

  156 pg/ml - 10,000 pg/ml

• Sensitivity:

  <10 pg/ml

• Function:

  Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities. .

• Components:

  Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

• Storage Conditions:

  Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

• Calculated Molecular Weight:

  63368 MW

• Specificity:

  Natural and recombinant human CD73

• Subcellular Location:

  Cell membrane; Lipid-anchor, GPI-anchor.

• Sequence Similarities:

  Belongs to the 5'-nucleotidase family.

• Protein Name:

  Ecto-5'-nucleotidase