Human MBL2/MPB-C ELISA Kit PicoKine®, Carrier-free

• Background:

  MBL2, also called mannose-binding lectin (protein C) 2, soluble or Mannose-binding lectin (MBL) is a lectin that is instrumental in innate immunity. MBL2 is mapped to chromosome 10q11.2-q21. It belongs to the class of collectins in the C-type lectin superfamily, whose function appears to be pattern recognition in the first line of defense in the pre-immune host. MBL2 recognizes carbohydrate patterns, found on the surface of a large number of pathogenic micro-organisms, including bacteria, viruses, protozoa and fungi. Binding MBL2 to a micro-organism results in activation of the lectin pathway of the complement system. Another important function of MBL2 is that this molecule binds senescent and apoptotic cells and enhances engulfment of whole, intact apoptotic cells, as well as cell debris by phagocytes.

• :

  heparin or EDTA

• Description:

  Human MBL2/MPB-C ELISA Kit PicoKine® (96 Tests). Quantitate Human MBL2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	479	546	475	475	493	30.21	6.1%
  Sample 2	2934	2910	2938	2814	2899	50.22	1.7%
  Sample 3	8702	9816	8527	9384	9107	519.57	5.7%

  *number of samples for each test n=16.

• Synonyms:

  Mannose-binding protein C;MBP-C;Collectin-1;MBP1;Mannan-binding protein;Mannose-binding lectin;MBL2;COLEC1, MBL;

• Gene Name:

  Mannose-binding protein C

• Gene ID:

  4153

• UniProt:

  P11226

• Reactivity:

  Human

• Cross Reactivity:

  There is no detectable cross-reactivity with other relevant proteins.

• Immunogen:

  Expression system for standard: NS0; Immunogen sequence: E21-I248

• Tissue Specificity:

  Plasma protein produced mainly in the liver. .

• Applications:

  ELISA

• Standard:

  20 ng/tube

• Sample Type:

  cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

• Detection Range:

  312 pg/ml - 20,000 pg/ml

• Sensitivity:

  <10 pg/ml

• Function:

  Calcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. May bind DNA. .

• Components:

  Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

• Storage Conditions:

  Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

• Calculated Molecular Weight:

  26144 MW

• Specificity:

  Natural and recombinant human MBP-C

• Subcellular Location:

  Secreted .

• Sequence Similarities:

  Contains 1 C-type lectin domain.

• Protein Name:

  Mannose-binding protein C