Human DKK-3 ELISA Kit PicoKine®, Carrier-free

• Background:

  Dickkopf-related protein 3 is a protein that in humans is encoded by the DKK3 gene. This gene encodes a protein that is a member of the dickkopf family. It is mapped to 11p15.3. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene. Members of the Dkk-related family display unique patterns of mRNA expression in human and mouse tissues, and are secreted when expressed in 293T cells. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.

• :

  heparin or EDTA

• Description:

  Human DKK-3 ELISA Kit PicoKine® (96 Tests). Quantitate Human DKK3 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	308	334	342	343	331	14.14	4.2%
  Sample 2	1957	1955	1972	1710	1898	109.02	5.7%
  Sample 3	5464	5826	5731	5903	5731	165.75	2.8%

  *number of samples for each test n=16.

• Synonyms:

  Dickkopf-related protein 3;Dickkopf-3;Dkk-3;hDkk-3;DKK3;REIC;UNQ258/PRO295;

• Gene Name:

  Dickkopf-related protein 3

• Gene ID:

  27122

• UniProt:

  Q9UBP4

• Reactivity:

  Human

• Cross Reactivity:

  There is no detectable cross-reactivity with other relevant proteins.

• Immunogen:

  Expression system for standard: NS0; Immunogen sequence: A22-I350

• Tissue Specificity:

  Highest expression in heart, brain, and spinal cord. .

• Applications:

  ELISA

• Standard:

  10 ng/tube

• Sample Type:

  cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

• Detection Range:

  156 pg/ml - 10,000 pg/ml

• Sensitivity:

  <10 pg/ml

• Function:

  Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease (By similarity). .

• Components:

  Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

• Storage Conditions:

  Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

• Calculated Molecular Weight:

  38390 MW

• Specificity:

  Natural and recombinant human DKK-3

• Subcellular Location:

  Secreted.

• Protein Name:

  Dickkopf-related protein 3