Mouse VEGFR3/FLT4 ELISA Kit PicoKine®, Carrier-free

• Background:

  Fms-related tyrosine kinase 4, also known as FLT4 or VEGFR3, is a protein which in humans is encoded by the FLT4 gene. It is mapped to 5q35.3. This gene encodes a tyrosine kinase receptor for vascular endothelial growth factors C and D. The protein is thought to be involved in lymphangiogenesis and maintenance of the lymphatic endothelium. FLT4 has an essential role in the development of the embryonic cardiovascular system before the emergence of the lymphatic vessels. It has been found that FLT4, which provides proangiogenic signaling when expressed on endothelium, may also have antiangiogenic properties when expressed at an avascular site by nonendothelial cells. FLT4 is also regarded as a regulator of vascular network formation.

• Description:

  Mouse VEGFR3/FLT4 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Flt4 in cell culture supernatants and serum. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	287	282	308	309	296	12.13	4%
  Sample 2	1392	1474	1350	1411	1406	44.66	3.1%
  Sample 3	5844	5515	6505	5591	5863	389.74	6.6%

  *number of samples for each test n=16.

• Synonyms:

  Vascular endothelial growth factor receptor 3;VEGFR-3;2.7.10.1;Fms-like tyrosine kinase 4;FLT-4;Tyrosine-protein kinase receptor FLT4;Flt4;Flt-4, Vegfr3;

• Gene Name:

  Vascular endothelial growth factor receptor 3

• Gene ID:

  14257

• UniProt:

  P35917

• Reactivity:

  Mouse

• Cross Reactivity:

  There is no detectable cross-reactivity with other relevant proteins.

• Immunogen:

  Expression system for standard: NS0; Immunogen sequence: Y25D770

• Tissue Specificity:

  Expressed in adult lung and liver, and in fetal liver, brain, intestine and placenta. .

• Applications:

  ELISA

• Standard:

  10 ng/tube

• Sample Type:

  cell culture supernatants and serum.

• Detection Range:

  156 pg/ml - 10,000 pg/ml

• Sensitivity:

  <10 pg/ml

• Function:

  Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFC and VEGFD, and plays an essential role in adult lymphangiogenesis and in the development of the vascular network and the cardiovascular system during embryonic development. Promotes proliferation, survival and migration of endothelial cells, and regulates angiogenic sprouting. Signaling by activated FLT4 leads to enhanced production of VEGFC, and to a lesser degree VEGFA, thereby creating a positive feedback loop that enhances FLT4 signaling. Modulates KDR signaling by forming heterodimers. Mediates activation of the MAPK1/ERK2, MAPK3/ERK1 signaling pathway, of MAPK8 and the JUN signaling pathway, and of the AKT1 signaling pathway. Phosphorylates SHC1. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3- kinase. Promotes phosphorylation of MAPK8 at 'Thr-183' and 'Tyr- 185', and of AKT1 at 'Ser-473'. .

• Components:

  Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

• Storage Conditions:

  Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

• Calculated Molecular Weight:

  153016 MW

• Specificity:

  Natural and recombinant mouse VEGFR3

• Subcellular Location:

  Cell membrane ; Single-pass type I membrane protein . Cytoplasm . Nucleus . Ligand-mediated autophosphorylation leads to rapid internalization.

• Sequence Similarities:

  Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.

• Protein Name:

  Vascular endothelial growth factor receptor 3