Mouse CD48/Slamf2 ELISA Kit PicoKine®, Carrier-free

• Background:

  CD48 antigen (Cluster of Differentiation 48), also known as BLAST-1 or SLAMF2, is a protein that in humans is encoded by the CD48 gene. CD48 is a member of the CD2 subfamily of the immunoglobulin superfamily (IgSF) which includes SLAM (signaling lymphocyte activation molecules) proteins, such as CD84, CD150, CD229 and CD244. It is mapped to 1q23.3. CD48 is found on the surface of lymphocytes and other immune cells, dendritic cells and endothelial cells, and participates in activation and differentiation pathways in these cells. The encoded protein does not have a transmembrane domain, however, but is held at the cell surface by a GPI anchor via a C-terminal domain which maybe cleaved to yield a soluble form of the receptor.

• :

  heparin or EDTA

• Description:

  Mouse CD48/Slamf2 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Cd48 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	91	79	95	86	87	5.97	6.8%
  Sample 2	816	795	773	755	784	22.93	2.9%
  Sample 3	1332	1482	1540	1536	1472	84.28	5.7%

  *number of samples for each test n=16.

• Synonyms:

  CD48 antigen;BCM1 surface antigen;BLAST-1;HM48-1;MRC OX-45 surface antigen;SLAM family member 2;SLAMF2;Signaling lymphocytic activation molecule 2;sgp-60;CD48;Cd48;Bcm-1;

• Gene Name:

  CD48 antigen

• Gene ID:

  12506

• UniProt:

  P18181

• Reactivity:

  Mouse

• Cross Reactivity:

  There is no detectable cross-reactivity with other relevant proteins.

• Immunogen:

  Expression system for standard: NS0; Immunogen sequence: F23-S217

• Applications:

  ELISA

• Standard:

  10 ng/tube

• Sample Type:

  cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

• Detection Range:

  62.5 pg/ml - 4,000 pg/ml

• Sensitivity:

  <10 pg/ml

• Function:

  Ligand for CD2. Might facilitate interaction between activated lymphocytes. Probably involved in regulating T-cell activation.

• Components:

  Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

• Storage Conditions:

  Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

• Calculated Molecular Weight:

  27383 MW

• Specificity:

  Natural and recombinant mouse CD48

• Subcellular Location:

  Cell membrane; Lipid-anchor, GPI-anchor.

• Sequence Similarities:

  Contains 2 Ig-like C2-type (immunoglobulin-like) domains.

• Protein Name:

  CD48 antigen