Mouse Lumican ELISA Kit PicoKine®, Carrier-free

Mouse Lumican ELISA Kit PicoKine®, Carrier-free

• Background: Lumican, also known as LUM, is a protein which in humans is encoded by the LUM gene. This gene is a member of the small interstitial proteoglycan gene (SIPG) family, and it is a keratan sulfate proteoglycan which presents large quantities in the corneal stroma and in interstitial collagenous matrices of the heart, aorta, skeletal muscle, skin, and intervertebral discs. Lumican is mapped to 12q21.33, it interacts with collagen and limits growth of fibrils in diameter. In the cornea, Lumican not only interacts with collagen molecules to limit fibril growth, but also plays a critical role in the regular spacing of fibrils and acquisition of corneal transparency by virtue of its keratan sulfate-containing glycosaminoglycan side chains LDC.
• Description: Mouse Lumican ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Lum in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
• Synonyms: Lumican;Keratan sulfate proteoglycan lumican;KSPG lumican;Lum;Lcn, Ldc;
• Gene Name: Lumican
• Gene ID: 17022
• UniProt: P51885
• Reactivity: Mouse
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: NS0; Immunogen sequence: Q19-N338
• Tissue Specificity: Cornea and other tissues.
• Applications: ELISA
• Standard: 10 ng/tube
• Sample Type: cell culture supernatants, serum and plasma (heparin).
• Detection Range: 156 pg/ml - 10,000 pg/ml
• Sensitivity: <10 pg/ml
• Components: Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4
• Storage Conditions: Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
• Specificity: Natural and recombinant mouse Lumican
• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	240	256	285	267	262	16.38	6.2%
  Sample 2	1132	1232	1167	1292	1205	61.37	5%
  Sample 3	5569	4938	5566	5489	5390	263.21	4.8%

  *number of samples for each test n=16.
• : heparin
• Calculated Molecular Weight: 38265 MW
• Subcellular Location: Secreted, extracellular space, extracellular matrix .
• Sequence Similarities: Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class II subfamily.
• Protein Name: Lumican