Human CCL23/MPIF-1 ELISA Kit PicoKine®, Carrier-free

• Background:

  Chemokine (C-C motif) ligand 23 (CCL23), also known as MIP-3 and MPIF-1, is a small cytokine belonging to the CC chemokine family. It is mapped to 17q12. CCL23 is predominantly expressed in lung and liver tissue, but is also found in placenta, bone marrow and some cell lines of myeloid origin. MPIF1 has chemotactic activity on resting T lymphocytes and monocytes, lower activity on neutrophils. It has also been attributed to an inhibitory activity on hematopoietic progenitor cells that give rise to granulocyte and monocyte lineages. Moreover, CCL23 is a ligand for the chemokine receptor CCR1.

• :

  heparin or EDTA

• Description:

  Human CCL23/MPIF-1 ELISA Kit PicoKine® (96 Tests). Quantitate Human CCL23 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	83	91	90	90	88	3.2	3.6%
  Sample 2	239	230	235	226	232	4.92	2.1%
  Sample 3	875	855	806	770	826	41.16	4.9%

  *number of samples for each test n=16.

• Synonyms:

  C-C motif chemokine 23;CK-beta-8;CKB-8;Macrophage inflammatory protein 3;MIP-3;Myeloid progenitor inhibitory factor 1;MPIF-1;Small-inducible cytokine A23;CCL23 (19-99);CCL23 (22-99);CCL23 (27-99);CCL23 (30-99);CCL23;MIP3, MPIF1, SCYA23;

• Gene Name:

  C-C motif chemokine 23

• Gene ID:

  6368

• UniProt:

  P55773

• Reactivity:

  Human

• Cross Reactivity:

  There is no detectable cross-reactivity with other relevant proteins.

• Immunogen:

  Expression system for standard: E.coli; Immunogen sequence: R22-N120

• Tissue Specificity:

  High levels in adult lung, liver, skeletal muscle and pancreas. Moderate levels in fetal liver, adult bone marrow and placenta. The short form is the major species and the longer form was detected only in very low abundance. CCL23 (19-99), CCL23 (22-99), CCL23 (27-99), CCL23 (30-99) are found in high levels in synovial fluids from rheumatoid patients. .

• Applications:

  ELISA

• Standard:

  10 ng/tube

• Sample Type:

  cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

• Detection Range:

  31.2 pg/ml - 2,000 pg/ml

• Sensitivity:

  <2 pg/ml

• Function:

  Shows chemotactic activity for monocytes, resting T- lymphocytes, and neutrophils, but not for activated lymphocytes. Inhibits proliferation of myeloid progenitor cells in colony formation assays. This protein can bind heparin. Binds CCR1. CCL23 (19-99), CCL23 (22-99), CCL23 (27-99), CCL23 (30-99) are more potent chemoattractants than the small-inducible cytokine A23. .

• Components:

  Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

• Storage Conditions:

  Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

• Calculated Molecular Weight:

  13411 MW

• Specificity:

  Natural and recombinant human CCL23

• Subcellular Location:

  Secreted.

• Sequence Similarities:

  Belongs to the intercrine beta (chemokine CC) family.

• Protein Name:

  C-C motif chemokine 23