Human CFD/Adipsin/Complement Factor D ELISA Kit PicoKine®, Carrier-free

Human CFD/Adipsin/Complement Factor D ELISA Kit PicoKine®, Carrier-free

• Background: Complement factor D (CFD), also called ADIPSIN or FACTOR D, is a protein which in humans is encoded by the CFD gene. The protein encoded by this gene belongs to the trypsin family of peptidases. It is mapped to 19p13.3. The encoded protein is a component of the alternative complement pathway which is best known for its role in humoral suppression of infectious agents. This protein is also a serine protease that is secreted by adipocytes into the bloodstream. Finally, the encoded protein has a high level of expression in fat, suggesting a role for adipose tissue in immune system biology. What's more, Factor D is a serine protease that stimulates glucose transport for triglyceride accumulation in fats cells and inhibits lipolysis.
• Description: Human CFD/Adipsin ELISA Kit PicoKine® (96 Tests). Quantitate Human CFD in cell culture supernatants, cell lysates, serum, plasma(heparin , EDTA) and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
• Synonyms: Complement factor D;3.4.21.46;Adipsin;C3 convertase activator;Properdin factor D;CFD;DF, PFD;
• Gene Name: Complement factor D
• Gene ID: 1675
• UniProt: P00746
• Reactivity: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: NS0; Immunogen sequence: I26-A253
• Applications: ELISA
• Standard: 20 ng/tube
• Sample Type: cell culture supernatants, cell lysates, serum, plasma(heparin , EDTA) and urine.
• Detection Range: 312 pg/ml - 20,000 pg/ml
• Sensitivity: <10 pg/ml
• Function: Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway.
• Components: Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4
• Storage Conditions: Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
• Specificity: Natural and recombinant human CFD
• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	787	751	817	732	771	32.75	4.2%
  Sample 2	2378	2440	2261	2414	2373	68.44	2.8%
  Sample 3	9095	10188	9364	8739	9346	534.02	5.7%

  *number of samples for each test n=16.
• : heparin or EDTA
• Calculated Molecular Weight: 27033 MW
• Subcellular Location: Secreted.
• Sequence Similarities: Belongs to the peptidase S1 family.
• Protein Name: Complement factor D