Human Endothelin ELISA Kit PicoKine®, Carrier-free

• Background:

  The endothelins is a family of structurally and pharmacologically distinct peptides, which has been identified and sequenced in humans. Three isoforms of human endothelin have been identified: endothelin-1, -2, and -3. Endothelin-1 is a potent, 21-amino acid vasoconstrictor peptide produced by vascular endothelial cells. Endothelins are 21-amino acid vasoconstricting peptides produced primarily in the endothelium having a key role in vascular homeostasis. Endothelins are implicated in vascular diseases of several organ systems, including the heart, general circulation and brain. Endothelins are proteins that constrict blood vessels and raise blood pressure. They are normally kept in balance by other mechanisms, but when they are over-expressed, they contribute to high blood pressure (hypertension) and heart disease.

• :

  heparin or EDTA

• Description:

  Human Endothelin ELISA Kit PicoKine® (96 Tests). Quantitate Human Endothelin in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 0.5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	11	13	12	14	12	1.11	9.2%
  Sample 2	51	49	47	47	48	1.65	3.4%
  Sample 3	131	117	110	124	120	7.82	6.5%

  *number of samples for each test n=16.

• Synonyms:

  Endothelin-1;Preproendothelin-1;PPET1;Endothelin-1;ET-1;Big endothelin-1;EDN1;

• Gene Name:

  endothelin 1, endothelin 2, endothelin 3

• Gene ID:

  1906, 1907, 1908

• UniProt:

  P05305, P20800, P14138

• Reactivity:

  Human

• Cross Reactivity:

  There is no detectable cross-reactivity with other relevant proteins.

• Immunogen:

  A peptide reflecting the target protein in human. Sequence: C53-W73

• Tissue Specificity:

  Expressed in lung, placental stem villi vessels and in cultured placental vascular smooth muscle cells. .

• Applications:

  ELISA

• Standard:

  1 ng/tube

• Sample Type:

  cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

• Detection Range:

  3.9 pg/ml - 250 pg/ml

• Sensitivity:

  <0.5 pg/ml

• Function:

  Endothelins are endothelium-derived vasoconstrictor peptides.

• Components:

  Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

• Storage Conditions:

  Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

• Calculated Molecular Weight:

  24425 MW

• Specificity:

  Natural and recombinant human Endothelin

• Subcellular Location:

  Secreted.

• Sequence Similarities:

  Belongs to the endothelin/sarafotoxin family.

• Protein Name:

  Endothelin-1, Endothelin-2, Endothelin-3