Human NOV/CCN3 ELISA Kit PicoKine®, Carrier-free

Human NOV/CCN3 ELISA Kit PicoKine®, Carrier-free

• Background: NOV (nephroblastoma overexpressed), also known as CCN3, is a matricellular protein that in humans is encoded by the NOV gene. The protein encoded by this gene is a small secreted cysteine-rich protein and a member of the CCN family of regulatory proteins. This gene is mapped to 8q24.12. NOV is a potentially useful marker for the diagnosis of adrenal gland diseases, malignant adrenocortical tumors, multiple sclerosis and so on. Moreover, reduced expression of NOV in ACTs may play an important role in the process of childhood ACT tumorigenesis. Though studying, it identified Nov as a regulator of human hematopoietic stem or progenitor cells.
• Description: Human NOV/CCN3 ELISA Kit PicoKine® (96 Tests). Quantitate Human CCN3 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
• Synonyms: Protein NOV homolog;NovH;CCN family member 3;Insulin-like growth factor-binding protein 9;IBP-9;IGF-binding protein 9;IGFBP-9;Nephroblastoma-overexpressed gene protein homolog;NOV;CCN3, IGFBP9, NOVH;
• Gene Name: Protein NOV homolog
• Gene ID: 4856
• UniProt: P48745
• Reactivity: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: NS0; Immunogen sequence: T32-M357
• Tissue Specificity: Expressed in bone marrow, thymic cells and nephroblastoma. Increased expression in Wilms tumor of the stromal type. .
• Applications: ELISA
• Standard: 10 ng/tube
• Sample Type: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
• Detection Range: 31.2 pg/ml - 2,000 pg/ml
• Sensitivity: <2 pg/ml
• Function: Immediate-early protein likely to play a role in cell growth regulation. .
• Components: Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4
• Storage Conditions: Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
• Specificity: Natural and recombinant human CCN3
• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	66	61	66	62	63	2.27	3.6%
  Sample 2	265	294	297	292	287	12.82	4.4%
  Sample 3	820	835	750	802	801	32.08	4%

  *number of samples for each test n=16.
• : heparin or EDTA
• Calculated Molecular Weight: 39162 MW
• Subcellular Location: Secreted.
• Sequence Similarities: Belongs to the CCN family.
• Protein Name: Protein NOV homolog