Mouse Adiponectin ELISA Kit PicoKine®, Carrier-free

• Background:

  Adiponectin (ADPN) is a hormone secreted by adipocytes that regulates energy homeostasis and glucose and lipid metabolism. Adiponectin is a new member of the family of soluble defense collagens, in hematopoiesis and immune responses. It is an important negative regulator in hematopoiesis and immune systems and raise the possibility that it may be involved in ending inflammatory responses through its inhibitory functions. Adiponectin is mapped to 3q27 and can protect the organism from systemic inflammation by promoting the clearance of early apoptotic cells by macrophages through a receptor-dependent pathway involving calreticulin.

• :

  heparin or EDTA

• Description:

  Mouse Adiponectin ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Adipoq in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	567	603	628	571	592	24.91	4.2%
  Sample 2	2994	2867	2845	2816	2880	67.97	2.3%
  Sample 3	7572	7510	8167	7413	7665	295.03	3.8%

  *number of samples for each test n=16.

• Synonyms:

  Adiponectin;30 kDa adipocyte complement-related protein;Adipocyte complement-related 30 kDa protein;ACRP30;Adipocyte, C1q and collagen domain-containing protein;Adipocyte-specific protein AdipoQ;Adipoq;Acdc, Acrp30, Apm1;

• Gene Name:

  Adiponectin

• Gene ID:

  11450

• UniProt:

  Q60994

• Reactivity:

  Mouse

• Cross Reactivity:

  There is no detectable cross-reactivity with other relevant proteins.

• Immunogen:

  Expression system for standard: NS0; Immunogen sequence: E18-N244

• Tissue Specificity:

  Synthesized exclusively by adipocytes and secreted into plasma.

• Applications:

  ELISA

• Standard:

  20 ng/tube

• Sample Type:

  cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

• Detection Range:

  312 pg/ml - 20,000 pg/ml

• Sensitivity:

  <10 pg/ml

• Function:

  Important adipokine involved in the control of fat metabolism and insulin sensitivity, with anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW. .

• Components:

  Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

• Storage Conditions:

  Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

• Calculated Molecular Weight:

  26809 MW

• Specificity:

  Natural and recombinant full length mouse Adiponectin

• Subcellular Location:

  Secreted.

• Sequence Similarities:

  Contains 1 C1q domain.

• Protein Name:

  Adiponectin