Mouse MMP-9 ELISA Kit PicoKine®, Carrier-free

• Background:

  The 92-kD type IV collagenase is also known as 92-kD gelatinase, type V collagenase, gelatinase B, or matrix metalloproteinase-9 (MMP9). The 72- and 92-kDa type IV collagenases are members of a group of secreted zinc metalloproteases.1 The matrix metalloproteinases (MMPs) are able to degrade the extracellular matrix and allow angiogenesis and tumor invasion. Gelatinase B, a matrix metalloproteinase that has proteolytic activity against connective tissue proteins, has been suggested to be important in the connective tissue remodeling processes associated with atherogenesis and plaque rupture. MMP-9 is predominantly expressed in neutrophils, macrophages, and mast cells, rather than in oncogene-positive neoplastic cells. The polymorphism of MMP-9 acts as a genetic factor for the development of smoking-induced pulmonary emphysema.

• :

  heparin

• Description:

  Mouse MMP-9 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Mmp9 in cell culture supernatants, serum and plasma ( heparin). Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	205	213	210	203	207	3.96	1.9%
  Sample 2	1787	1777	1813	1705	1770	40.03	2.2%
  Sample 3	4289	3856	3877	4024	4011	172.79	4.3%

  *number of samples for each test n=16.

• Synonyms:

  Matrix metalloproteinase-9;MMP-9;3.4.24.35;92 kDa gelatinase;92 kDa type IV collagenase;Gelatinase B;GELB;Mmp9;Clg4b;

• Gene Name:

  Matrix metalloproteinase-9

• Gene ID:

  17395

• UniProt:

  P41245

• Reactivity:

  Mouse

• Cross Reactivity:

  There is no detectable cross-reactivity with other relevant proteins.

• Immunogen:

  Expression system for standard: CHO; Immunogen sequence: A20-P730

• Applications:

  ELISA

• Standard:

  10 ng/tube

• Sample Type:

  cell culture supernatants, serum and plasma ( heparin).

• Detection Range:

  156 pg/ml - 10,000 pg/ml

• Sensitivity:

  <20 pg/ml

• Function:

  Could play a role in bone osteoclastic resorption. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments (By similarity). .

• Components:

  Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

• Storage Conditions:

  Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

• Calculated Molecular Weight:

  80535 MW

• Specificity:

  Natural and recombinant mouse total MMP-9

• Subcellular Location:

  Secreted, extracellular space, extracellular matrix .

• Sequence Similarities:

  Belongs to the peptidase M10A family.

• Protein Name:

  Matrix metalloproteinase-9