Mouse IL-4/Interleukin-4 ELISA Kit PicoKine®, Carrier-free

• Background:

  Interleukin-4 (IL-4), also knowns as a B-cell stimulatory factor1 (BSF1), is an immunomodulatory cytokine, which can inhibit the growth of tumour cells. The human cDNA contains a single open reading frame encoding a protein of 153 amino acids, including a putative signal peptide. IL-4 may act as an autocrine growth factor in pancreatic cancer cells and also give rise to the possibility that cancer-derived IL-4 may suppress cancer-ed immunosurveillance in vivo in addition to its growth-promoting effects, thereby facilitating pancreatic tumor growth and metastasis.1 The mouse and human genes and their protein products show structural and functional similarities. The human IL-4 gene, which occurs as a single copy in the haploid genome, is mapped on chromosome 5.

• Description:

  Mouse IL-4/Interleukin-4 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Il4 in cell culture supernatants and serum. Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	15	14	13	16	14	1.11	7.9%
  Sample 2	96	90	93	94	93	2.16	2.3%
  Sample 3	280	285	256	248	267	15.61	5.8%

  *number of samples for each test n=16.

• Synonyms:

  Interleukin-4;IL-4;B-cell IgG differentiation factor;B-cell growth factor 1;B-cell stimulatory factor 1;BSF-1;IGG1 induction factor;Lymphocyte stimulatory factor 1;Il4;Il-4;

• Gene Name:

  Interleukin-4

• Gene ID:

  16189

• UniProt:

  P07750

• Reactivity:

  Mouse

• Cross Reactivity:

  There is no detectable cross-reactivity with other relevant proteins.

• Immunogen:

  Expression system for standard: E.coli; Immunogen sequence: H21-S140

• Applications:

  ELISA

• Standard:

  10 ng/tube

• Sample Type:

  cell culture supernatants and serum.

• Detection Range:

  7.8 pg/ml - 500 pg/ml

• Sensitivity:

  <1 pg/ml

• Function:

  Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes.

• Components:

  Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

• Storage Conditions:

  Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

• Calculated Molecular Weight:

  15834 MW

• Specificity:

  Natural and recombinant mouse IL-4

• Subcellular Location:

  Secreted.

• Protein Name:

  Interleukin-4