Mouse CD117/C-Kit PicoKine® Quick ELISA Kit, Carrier-free

• Background:

  SCFR(Mast/stem cell growth factor receptor), also known as proto-oncogene c-Kit or tyrosine-protein kinase Kit or CD117, is a protein that in humans is encoded by the KIT gene. KIT was first described as the cellular homolog of the feline sarcoma viral oncogene v-kit. The KIT gene is mapped on 4q12. Kit was expressed on the surface of germ cells up to the pachytene stage. Signaling from the KIT receptor tyrosine kinase is essential for primordial germ cell growth both in vivo and in vitro. Determination of the KIT effectors acting in primordial germ cells has been hampered by the lack of effective methods to manipulate easily gene expression in these cells.

• :

  heparin or EDTA

• Description:

  Mouse CD117/C-Kit PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Mouse CD117/C-Kit in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

• Reproducibility:

  Reproducibility

  To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	242	256	258	231	247	11.1	4.5%
  Sample 2	1796	1755	1641	1630	1706	110.86	6.5%
  Sample 3	5641	5751	6321	6874	6147	270.46	4.4%

  *number of samples for each test n=16.

• Gene Name:

  Kit

• Gene ID:

  16590

• UniProt:

  P05532

• Reactivity:

  Mouse

• Cross Reactivity:

  There is no detectable cross-reactivity with other relevant proteins.

• Immunogen:

  Expression system for standard: NS0; Immunogen sequence: S25-P527

• Applications:

  ELISA

• Standard:

  10 ng/tube

• Sample Type:

  cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

• Detection Range:

  156 pg/ml - 10,000 pg/ml

• Sensitivity:

  <10 pg/ml

• Components:

  Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x)|1|12ml Color Developing Reagent (TMB)|1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

• Storage Conditions:

  Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

• Specificity:

  Natural and recombinant Mouse Kit