Human Decorin / DCN PicoKine® Quick ELISA Kit, Carrier-free

• Background:

  Decorin is a proteoglycan on average 90 - 140 kilodaltons (kD) in size.It belongs to the small leucine-rich proteoglycan (SLRP) family and consists of a protein core containing leucine repeats with a glycosaminoglycan (GAG) chain consisting of either chondroitin sulfate (CS) or dermatan sulfate (DS).Using a genomic clone as the labeled probe and in situ hybridization of human metaphase chromosomes, decorin gene was mapped to the discrete region of human chromosome 12q23. Decorin is capable of suppressing the growth of various tumor cell lines when expressed ectopically.

• :

  heparin or EDTA

• Description:

  Human Decorin / DCN PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Human DCN in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	60	51	50	59	55	4.52	8.2%
  Sample 2	247	279	302	287	278	20.1	7.2%
  Sample 3	1001	962	922	976	965	28.61	2.9%

  *number of samples for each test n=16.

• Synonyms:

  Decorin;Bone proteoglycan II;PG-S2;PG40;DCN;SLRR1B;

• Gene Name:

  Decorin

• Gene ID:

  1634

• UniProt:

  P07585

• Reactivity:

  Human

• Cross Reactivity:

  There is no detectable cross-reactivity with other relevant proteins.

• Immunogen:

  Expression system for standard: NS0; Immunogen sequence: D31-K359

• Applications:

  ELISA

• Standard:

  10 ng/tube

• Sample Type:

  cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

• Detection Range:

  31.2 pg/ml - 2,000 pg/ml

• Sensitivity:

  <10 pg/ml

• Function:

  May affect the rate of fibrils formation.

• Components:

  Description|Quantity 96-well plate|1 lyophilized Standard| Biotinylated detection antibody (50x)|100 ul Avidin-Biotin-Peroxidase Complex (30x)|400 µl Sample diluent|30 ml Antibody diluent|12 ml Avidin-Biotin-Peroxidase Diluent|12 ml Wash Buffer (25x)|20 ml Color Developing Reagent (TMB)|10 ml Stop Solution|10 ml Plate Sealers|4

• Storage Conditions:

  Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

• Calculated Molecular Weight:

  39747 MW

• Specificity:

  Natural and recombinant human Decorin

• Subcellular Location:

  Secreted, extracellular space, extracellular matrix.

• Sequence Similarities:

  Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.

• Protein Name:

  Decorin