Human IL-5/Interleukin-5 PicoKine® Quick ELISA Kit, Carrier-free

• Background:

  Interleukin-5 (IL-5) is also known as eosinophil differentiation factor (EDF). IL-5 is a potential candidate gene in the pathogenesis of asthma, as it is the main cytokine controlling eosinophil activity and eosinophils are pivotal in the development of airway inflammation. The predicted amino acid sequence of 134 amino acids is identical with that recently reported for human interleukin-5 but shows no significant homology with other known hemopoietic growth regulators. IL-5 is a lineage-specific hematopoietic growth factor that stimulates the production of eosinophils and eosinophil colonies from normal human bone marrow cells. IL-5 gene to human chromosome 5 at bands q23-31. Human and mouse of IL-5 share 70% amino acid sequence homology.

• :

  heparin or EDTA

• Description:

  Human IL-5/Interleukin-5 PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Human IL5 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	9	10	10	9	9	0.5	5.5%
  Sample 2	73	73	74	62	70	4.92	7%
  Sample 3	238	246	219	235	234	9.81	4.1%

  *number of samples for each test n=16.

• Synonyms:

  Interleukin-5;IL-5;B-cell differentiation factor I;Eosinophil differentiation factor;T-cell replacing factor;TRF;IL5;

• Gene Name:

  Interleukin-5

• Gene ID:

  3567

• UniProt:

  P05113

• Reactivity:

  Human

• Cross Reactivity:

  There is no detectable cross-reactivity with other relevant proteins.

• Immunogen:

  Expression system for standard: NS0; Immunogen sequence: I20-S134

• Applications:

  ELISA

• Standard:

  10 ng/tube

• Sample Type:

  cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

• Detection Range:

  7.8 pg/ml - 500 pg/ml

• Sensitivity:

  <2 pg/ml

• Function:

  Factor that induces terminal differentiation of late- developing B-cells to immunoglobulin secreting cells.

• Components:

  Description|Quantity 96-well plate|1 lyophilized Standard| Biotinylated detection antibody (50x)|100 ul Avidin-Biotin-Peroxidase Complex (30x)|400 µl Sample diluent|30 ml Antibody diluent|12 ml Avidin-Biotin-Peroxidase Diluent|12 ml Wash Buffer (25x)|20 ml Color Developing Reagent (TMB)|10 ml Stop Solution|10 ml Plate Sealers|4

• Storage Conditions:

  Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

• Calculated Molecular Weight:

  15238 MW

• Specificity:

  Natural and recombinant human IL-5

• Subcellular Location:

  Secreted.

• Sequence Similarities:

  Belongs to the IL-5 family.

• Protein Name:

  Interleukin-5