Citrate Buffer Powder, Carrier-free

Citrate Buffer Powder, Carrier-free

• Background: In immunohistochemistry (IHC) the most commonly used fixatives for preserving cell morphology and tissue architecture cause cross-linkages that mask epitopes, leading to poor or no staining. To reverse the loss of antigenicity and enable the primary antibodies to bind antigens, Heat-Induced Epitope Retrieval (HIER) is used to unmask epitopes by breaking the methylene bridges formed during fixation. During the process of HIER the buffer helps unfold the proteins, improving the accessibility of antibodies to tissue antigens. Citrate buffer solution is the preferred antigen retrieval buffer for most antibodies during HIER.
• Description: Boster’s Citrate Buffer Pack is an IHC antigen retrieval reagent used in heat induced epitope retrieval (HIER) for recovery of antigens masked by cross-linking during fixation.
• Storage Conditions: Store at room temperature (RT) for one year
• Method: See the IHC protocol for more specific usage instructions. Certain antigens may utilize a combination of retrieval methods. Antigen retrieval may not be required for frozen cross section as cross links are formed during formalin fixation. Advice: In order to achieve sufficient retrieval or avoid tissue destruction or over digestion, appropriate temperature, pH, buffer concentration, incubation times, and other conditions should be carefully selected for each particular antigen, antibody, or tissue type after preliminary experimentation by the researcher. Note: All of Boster's products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use.