Anti-Collagen III Rabbit Monoclonal Antibody, Carrier-free

• Description:

  Boster Bio Anti-Collagen III Rabbit Monoclonal Antibody catalog # M00788-1. Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

• Synonyms:

  Estrogen receptor beta;ER-beta;Nuclear receptor subfamily 3 group A member 2;ESR2;ESTRB, NR3A2;

• Gene Name:

  Estrogen receptor beta

• UniProt:

  P02461

• Host:

  Rabbit

• Reactivity:

  Human,Mouse,Rat

• Immunogen:

  A synthesized peptide derived from Collagen III

• Clonality:

  Monoclonal

• Clone:

  Clone: 26C74

• Tissue Specificity:

  Isoform beta-1 is expressed in testis and ovary, and at a lower level in heart, brain, placenta, liver, skeletal muscle, spleen, thymus, prostate, colon, bone marrow, mammary gland and uterus. Also found in uterine bone, breast, and ovarian tumor cell lines, but not in colon and liver tumors. Isoform beta-2 is expressed in spleen, thymus, testis and ovary and at a lower level in skeletal muscle, prostate, colon, small intestine, leukocytes, bone marrow, mammary gland and uterus. Isoform beta-3 is found in testis. Isoform beta-4 is expressed in testis, and at a lower level in spleen, thymus, ovary, mammary gland and uterus. Isoform beta-5 is expressed in testis, placenta, skeletal muscle, spleen and leukocytes, and at a lower level in heart, lung, liver, kidney, pancreas, thymus, prostate, colon, small intestine, bone marrow, mammary gland and uterus. Not expressed in brain.

• Applications:

  WB,ICC,IF,IP,Flow Cytometry

• Purification:

  Affinity-chromatography

• Concentration:

  Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

• Form:

  Liquid

• Reconstitution:

  Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

• Function:

  Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner (PubMed:20074560). Isoform beta-cx lacks ligand binding ability and has no or only very low ere binding activity resulting in the loss of ligand-dependent transactivation ability. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual. .

• Components:

  Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

• Storage Conditions:

  Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  59216 MW

• Observed Molecular Weight:

  150 kDa-200 kDa

• Fragment:

  IgG

• Applications Notes:

  WB 1:500-1:2000
  ICC/IF 1:50-1:200
  IP 1:50
  FC 1:50

• Subcellular Location:

  Nucleus .

• Protein Name:

  T-cell surface glycoprotein CD4