Anti-Smad2 Rabbit Monoclonal Antibody, Carrier-free

• Description:

  Boster Bio Anti-Smad2 Rabbit Monoclonal Antibody catalog # M00090. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Rat.

• Synonyms:

  Mothers against decapentaplegic homolog 2;MAD homolog 2;Mothers against DPP homolog 2;JV18-1;Mad-related protein 2;hMAD-2;SMAD family member 2;SMAD 2;Smad2;hSMAD2;SMAD2;MADH2, MADR2;

• Gene Name:

  Mothers against decapentaplegic homolog 2

• UniProt:

  Q15796

• Host:

  Rabbit

• Reactivity:

  Human,Rat

• Immunogen:

  A synthesized peptide derived from human Smad2

• Clonality:

  Monoclonal

• Clone:

  Clone: EFF-19

• Tissue Specificity:

  Expressed at high levels in skeletal muscle, endothelial cells, heart and placenta. .

• Applications:

  WB,IHC,ICC,IF,IP,Flow Cytometry

• Purification:

  Affinity-chromatography

• Concentration:

  Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

• Form:

  Liquid

• Reconstitution:

  Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

• Function:

  Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. .

• Components:

  Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

• Storage Conditions:

  Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  52306 MW

• Observed Molecular Weight:

  75 kDa

• Fragment:

  Rabbit IgG

• Applications Notes:

  WB 1:1000-1:2000
  IHC 1:50-1:200
  ICC/IF 1:50-1:200
  IP 1:50
  FC 1:50

• Subcellular Location:

  Cytoplasm. Nucleus. Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4. On dephosphorylation by phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1.

• Protein Name:

  P2X purinoceptor 1