Anti-NUPL2/NUP42 Antibody Picoband®, Carrier-free

• Background:

  NUPL2 is required for the export of mRNAs containing poly(A) tails from the nucleus into the cytoplasm. In case of infection by HIV-1, it may participate in the docking of viral Vpr at the nuclear envelope.

• Description:

  Boster Bio Anti-NUPL2/NUP42 Antibody Picoband® catalog # A09480. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Synonyms:

  Mediator of RNA polymerase II transcription subunit 20; Mediator complex subunit 20; TRF-proximal protein homolog; hTRFP; MED20; TRFP;

• Gene Name:

  CD72 molecule

• Gene ID:

  11097

• UniProt:

  O15504

• Host:

  Rabbit

• Reactivity:

  Human,Mouse,Rat

• Cross Reactivity:

  No cross-reactivity with other proteins.

• Immunogen:

  E.coli-derived human NUPL2/NUP42 recombinant protein (Position: Q52-V423).

• Clonality:

  Polyclonal

• Tissue Specificity:

  Pre-B-cells and B-cells but not terminally differentiated plasma cells.

• Applications:

  WB,ELISA

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Function:

  Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

• Components:

  Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

• References & Citations:

  1. Farjot, G., Sergeant, A., Mikaelian, I. A new nucleoporin-like protein interacts with both HIV-1 Rev nuclear export signal and CRM-1. J. Biol. Chem. 274: 17309-17317, 1999. 2. Gross, M. B. Personal Communication. Baltimore, Md. 8/11/2022. 3. Takahashi, Y., Mizoi, J., Toh-E, A., Kikuchi, Y. Yeast Ulp1, an Smt3-specific protease, associates with nucleoporins. J. Biochem. 128: 723-725, 2000.

• Storage Conditions:

  At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

• Observed Molecular Weight:

  36 kDa

• Fragment:

  Rabbit IgG

• Applications Notes:

  Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
  ELISA, 0.1-0.5 μg/ml, -
  

• Subcellular Location:

  Nucleus .