Anti-Ribonuclease H2, subunit A/RNASEH2A Antibody Picoband®, Carrier-free

Anti-Ribonuclease H2, subunit A/RNASEH2A Antibody Picoband®, Carrier-free

• Background: Ribonuclease H2 subunit A, also known as RNase H2 subunit A, is an enzyme that in humans is encoded by the RNASEH2A gene. The protein encoded by this gene is a component of the heterotrimeric type II ribonuclease H enzyme (RNAseH2). RNAseH2 is the major source of ribonuclease H activity in mammalian cells and endonucleolytically cleaves ribonucleotides. It is predicted to remove Okazaki fragment RNA primers during lagging strand DNA synthesis and to excise single ribonucleotides from DNA-DNA duplexes. Mutations in this gene cause Aicardi-Goutieres Syndrome (AGS), a an autosomal recessive neurological disorder characterized by progressive microcephaly and psychomotor retardation, intracranial calcifications, elevated levels of interferon-alpha and white blood cells in the cerebrospinal fluid.
• Description: Boster Bio Anti-Ribonuclease H2, subunit A/RNASEH2A Antibody Picoband® catalog # A07386-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
• Synonyms: Mucin-20; MUC-20; MUC20; KIAA1359; UNQ2782/PRO7170
• Gene Name: mucin 20, cell surface associated
• Gene ID: 10535
• UniProt: O75792
• Host: Rabbit
• Reactivity: Human
• Cross Reactivity: No cross-reactivity with other proteins.
• Immunogen: E.coli-derived human Ribonuclease H2, subunit A/RNASEH2A recombinant protein (Position: R25-L299).
• Clonality: Polyclonal
• Tissue Specificity: Highly expressed in kidney, moderately in placenta, lung, prostate, liver, and digestive system. In the kidney, localized in the proximal tubules but not in the glomerulus or distal tubules. Detected in most of the male urogenital tract epithelia, with the exception of epididymis.
• Applications: WB,Flow Cytometry,ELISA
• Purification: Immunogen affinity purified.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form: Lyophilized
• Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Function: May regulate MET signaling cascade. Seems to decrease hepatocyte growth factor (HGF)-induced transient MAPK activation. Blocks GRB2 recruitment to MET thus suppressing the GRB2-RAS pathway. Inhibits HGF-induced proliferation of MMP1 and MMP9 expression.
• Components: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
• References & Citations: 1. Coffin, S. R., Hollis, T., Perrino, F. W. Functional consequences of the RNase H2A subunit mutations that cause Aicardi-Goutieres syndrome. J. Biol. Chem. 286: 16984-16991, 2011. 2. Crow, Y. J., Leitch, A., Hayward, B. E., Garner, A., Parmar, R., Griffith, E., Ali, M., Semple, C., Aicardi, J., Babul-Hirji, R., Baumann, C., Baxter, P., and 33 others. Mutations in genes encoding ribonuclease H2 subunits cause Aicardi-Goutieres syndrome and mimic congenital viral brain infection. Nature Genet. 38: 910-916, 2006. 3. Frank, P., Braunshofer-Reiter, C., Wintersberger, U., Grimm, R., Busen, W. Cloning of the cDNA encoding the large subunit of human RNase HI, a homologue of the prokaryotic RNase HII. Proc. Nat. Acad. Sci. 95: 12872-12877, 1998.
• Storage Conditions: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.
• Calculated Molecular Weight: 108320 MW
• Observed Molecular Weight: 33 kDa
• Fragment: Rabbit IgG
• Applications Notes: Western blot, 0.25-0.5 μg/ml, Human
  Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
  ELISA, 0.1-0.5 μg/ml, -
  
• Subcellular Location: Apical cell membrane. Basolateral cell membrane. Microvillus membrane. Secreted.
• Protein Name: Gamma-aminobutyric acid type B receptor subunit 1