Anti-LXR beta/NER/NR1H2 Antibody Picoband®, Carrier-free

Anti-LXR beta/NER/NR1H2 Antibody Picoband®, Carrier-free

• Background: Liver X receptor beta (LXR-β) is a member of the nuclear receptor family of transcription factors. LXR-β is encoded by the NR1H2 gene (nuclear receptor subfamily 1, group H, member 2). The liver X receptors, LXRA (NR1H3; MIM 602423) and LXRB, form a subfamily of the nuclear receptor superfamily and are key regulators of macrophage function, controlling transcriptional programs involved in lipid homeostasis and inflammation. The inducible LXRA is highly expressed in liver, adrenal gland, intestine, adipose tissue, macrophages, lung, and kidney, whereas LXRB is ubiquitously expressed. Ligand-activated LXRs form obligate heterodimers with retinoid X receptors (RXRs; see MIM 180245) and regulate expression of target genes containing LXR response elements (summary by Korf et al., 2009 [PubMed 19436111]).
• Description: Boster Bio Anti-LXR beta/NER/NR1H2 Antibody Picoband® catalog # A04523-2. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
• Synonyms: Vascular endothelial growth factor B; VEGF-B; VEGF-related factor; VRF; VEGFB; VRF
• Gene Name: vascular endothelial growth factor B
• Gene ID: 7376
• UniProt: P55055
• Host: Rabbit
• Reactivity: Human,Mouse
• Cross Reactivity: No cross-reactivity with other proteins.
• Immunogen: E.coli-derived human LXR beta/NER/NR1H2 recombinant protein (Position: K31-A342).
• Clonality: Polyclonal
• Tissue Specificity: Expressed in all tissues except liver. Highest levels found in heart, skeletal muscle and pancreas.
• Applications: ELISA,Flow Cytometry,IF,ICC,WB
• Purification: Immunogen affinity purified.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form: Lyophilized
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
• Function: Growth factor for endothelial cells. VEGF-B167 binds heparin and neuropilin-1 whereas the binding to neuropilin-1 of VEGF-B186 is regulated by proteolysis.
• Components: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.005mg NaN3.
• References & Citations: 1. Andersson, S., Gustafsson, N., Warner, M., Gustafsson, J.-A. Inactivation of liver X receptor beta leads to adult-onset motor neuron degeneration in male mice. Proc. Nat. Acad. Sci. 102: 3857-3862, 2005. Note: Erratum: Proc. Nat. Acad. Sci. 103: 8298 only, 2006. 2. Bensinger, S. J., Bradley, M. N., Joseph, S. B., Zelcer, N., Janssen, E. M., Hausner, M. A., Shih, R., Parks, J. S., Edwards, P. A., Jamieson, B. D., Tontonoz, P. LXR signaling couples sterol metabolism to proliferation in the acquired immune response. Cell 134: 97-111, 2008. 3. Bradley, M. N., Hong, C., Chen, M., Joseph, S. B., Wilpitz, D. C., Wang, X., Lusis, A. J., Collins, A., Hseuh, W. A., Collins, J. L., Tangirala, R. K., Tontonoz, P. Ligand activation of LXR-beta reverses atherosclerosis and cellular cholesterol overload in mice lacking LXR-alpha and apoE. J. Clin. Invest. 117: 2337-2346, 2007.
• Storage Conditions: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
• Observed Molecular Weight: 50 kDa
• Fragment: Rabbit IgG
• Applications Notes: Western blot, 0.25-0.5μg/ml, Human, Mouse
  Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
  Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human
  ELISA, 0.1-0.5μg/ml, -
  
• Subcellular Location: Secreted. Secreted but remains associated to cells or to the extracellular matrix unless released by heparin.