Anti-DHX15/prp43 Antibody Picoband®, Carrier-free

• Background:

  Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15 is an enzyme that in humans is encoded by the DHX15 gene. It is mapped to 4p15.2. The protein encoded by this gene is a putative ATP-dependent RNA helicase implicated in pre-mRNA splicing.

• Description:

  Boster Bio Anti-DHX15/prp43 Antibody Picoband® catalog # A08140-1. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Synonyms:

  Pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15; ATP-dependent RNA helicase #46; DEAH box protein 15; DHX15; DBP1, DDX15

• Gene Name:

  DEAH-box helicase 15

• Gene ID:

  1665

• UniProt:

  O43143

• Host:

  Rabbit

• Reactivity:

  Human,Mouse,Rat

• Cross Reactivity:

  No cross-reactivity with other proteins.

• Immunogen:

  A synthetic peptide corresponding to a sequence at the C-terminus of human DHX15/prp43, identical to the related mouse and rat sequences.

• Clonality:

  Polyclonal

• Tissue Specificity:

  Ubiquitous.

• Applications:

  IF,IHC,ICC,WB,Flow Cytometry

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

• Function:

  Pre-mRNA processing factor involved in disassembly of spliceosomes after the release of mature mRNA. In cooperation with TFIP11 seem to be involved in the transition of the U2, U5 and U6 snRNP-containing IL complex to the snRNP-free IS complex leading to efficient debranching and turnover of excised introns.

• Components:

  Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

• References & Citations:

  1. Lüking A, Stahl U, Schmidt U (1998). "The protein family of RNA helicases". Crit. Rev. Biochem. Mol. Biol. 33 (4): 259–96. 2. Will CL, Schneider C, Hossbach M, et al. (2004). "The human 18S U11/U12 snRNP contains a set of novel proteins not found in the U2-dependent spliceosome". RNA. 10 (6): 929–41. 3. Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5.

• Storage Conditions:

  Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  17136 MW

• Observed Molecular Weight:

  91 kDa

• Fragment:

  Rabbit IgG

• Specificity:

  No cross reactivity with other proteins.

• Applications Notes:

  Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
  Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human
  Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
  Flow Cytometry(Fixed), 1-3μg/1x10⁶ cells, Human
  

• Subcellular Location:

  Postsynaptic density. Cytoskeleton. Cytoplasm. Axon. Dendrite. Synapse.

• Protein Name:

  Chromaffin granule amine transporter