Anti-Acetyl Coenzyme A Carboxylase/ACACB Antibody Picoband®, Carrier-free

• Background:

  Acetyl-CoA carboxylase 2 also known as ACC-beta or ACC2 is an enzyme that in humans is encoded by the ACACB gene. It is mapped to 12q24.11. Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. ACC-beta is thought to control fatty acid oxidation by means of the ability of malonyl-CoA to inhibit carnitine-palmitoyl-CoA transferase I, the rate-limiting step in fatty acid uptake and oxidation by mitochondria. ACC-beta may be involved in the regulation of fatty acid oxidation, rather than fatty acid biosynthesis. There is evidence for the presence of two ACC-beta isoforms.

• Description:

  Boster Bio Anti-Acetyl Coenzyme A Carboxylase/ACACB Antibody Picoband® catalog # A03668-2. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Synonyms:

  Acetyl-CoA carboxylase 2; ACC-beta; Biotin carboxylase; ACACB; ACC2, ACCB

• Gene Name:

  acetyl-CoA carboxylase beta

• Gene ID:

  32

• UniProt:

  O00763

• Host:

  Rabbit

• Reactivity:

  Human,Mouse,Rat

• Cross Reactivity:

  No cross-reactivity with other proteins.

• Immunogen:

  A synthetic peptide corresponding to a sequence at the C-terminus of human Acetyl Coenzyme A Carboxylase/ACACB, which shares 64.7% amino acid (aa) sequence identity with mouse Acetyl Coenzyme A Carboxylase/ACACB.

• Clonality:

  Polyclonal

• Tissue Specificity:

  Widely expressed with highest levels in heart, skeletal muscle, liver, adipose tissue, mammary gland, adrenal gland and colon (PubMed:9099716). Isoform 3 is expressed in skeletal muscle, adipose tissue and liver (at protein level) (PubMed:19190759). Isoform 3 is detected at high levels in adipose tissue with lower levels in heart, liver, skeletal muscle and testis (PubMed:19190759).

• Applications:

  Flow Cytometry,IHC,WB

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

• Function:

  Catalyzes the ATP-dependent carboxylation of acetyl-CoA to malonyl-CoA (PubMed:20457939, PubMed:19236960, PubMed:20952656). Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase (PubMed:20457939, PubMed:19236960, PubMed:20952656). Involved in inhibition of fatty acid and glucose oxidation and enhancement of fat storage (By similarity). May play a role in regulation of mitochondrial fatty acid oxidation through malonyl-CoA-dependent inhibition of carnitine palmitoyltransferase 1 (By similarity).

• Components:

  Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

• References & Citations:

  1. Widmer J, Fassihi KS, Schlichter SC, Wheeler KS, Crute BE, King N, Nutile-McMenemy N, Noll WW, Daniel S, Ha J, Kim KH, Witters LA (June 1996). "Identification of a second human acetyl-CoA carboxylase gene". The Biochemical Journal. 316 ( Pt 3) (3): 915–22. 2. Corbett JW, Harwood JH (November 2007). "Inhibitors of mammalian acetyl-CoA carboxylase". Recent Patents on Cardiovascular Drug Discovery. 2 (3): 162–80. 3. Olson DP, Pulinilkunnil T, Cline GW, Shulman GI, Lowell BB (April 2010). "Gene knockout of Acc2 has little effect on body weight, fat mass, or food intake". Proceedings of the National Academy of Sciences of the United States of America. 107 (16): 7598–603.

• Storage Conditions:

  Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  28461 MW

• Observed Molecular Weight:

  277 kDa

• Fragment:

  Rabbit IgG

• Specificity:

  No cross reactivity with other proteins.

• Applications Notes:

  Western blot, 0.25-0.5μg/ml, Mouse, Rat
  Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, By Heat
  Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human
  

• Subcellular Location:

  Nucleus. Cytosol.

• Protein Name:

  Breast cancer metastasis-suppressor 1