Anti-Human CCR3 DyLight® 550 conjugated Antibody, Carrier-free

• Background:

  C-C chemokine receptor type 3, also called CCR3 or CKR3 is a protein that in humans is encoded by the CCR3 gene. The protein encoded by this gene is a receptor for C-C type chemokines. It belongs to family 1 of the G protein-coupled receptors. This gene and seven other chemokine receptor genes form a chemokine receptor gene cluster on the chromosomal region 3p21. This receptor binds and responds to a variety of chemokines, including eotaxin (CCL11), eotaxin-3 (CCL26), MCP-3 (CCL7), MCP-4 (CCL13), and RANTES (CCL5). It is highly expressed in eosinophils and basophils, and is also detected in TH1 and TH2 cells, as well as in airway epithelial cells. This receptor may contribute to the accumulation and activation of eosinophils and other inflammatory cells in the allergic airway. It is also known to be an entry co-receptor for HIV-1. Alternatively spliced transcript variants have been described.

• Description:

  Boster Bio Anti-Human CCR3 DyLight® 550 conjugated Antibody catalog # A01748-Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Human.

• Synonyms:

  C-C chemokine receptor type 3; C-C CKR-3; CC-CKR-3; CCR-3; CCR3; CKR3; Eosinophil eotaxin receptor; CD193; CCR3; CMKBR3

• Gene Name:

  chemokine (C-C motif) receptor 3

• Gene ID:

  1232

• UniProt:

  P51677

• Host:

  Rabbit

• Reactivity:

  Human

• Cross Reactivity:

  No cross-reactivity with other proteins.

• Immunogen:

  A synthetic peptide corresponding to a sequence at the N-terminus of human CCR3.

• Clonality:

  Polyclonal

• Label:

  DyLight®550

• Tissue Specificity:

  In eosinophils as well as trace amounts in neutrophils and monocytes.

• Applications:

  Flow Cytometry

• Concentration:

  0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

• Form:

  Liquid

• Function:

  Receptor for a C-C type chemokine. Binds to eotaxin, eotaxin-3, MCP-3, MCP-4, RANTES and MIP-1 delta. Subsequently transduces a signal by increasing the intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection.

• Components:

  Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.

• References & Citations:

  1. Blanchard, C., Wang, N., Stringer, K. F., Mishra, A., Fulkerson, P. C., Abonia, J. P., Jameson, S. C., Kirby, C., Konikoff, M. R., Collins, M. H., Cohen, M. B., Akers, R., Hogan, S. P., Assa'ad, A. H., Putnam, P. E., Aronow, B. J., Rothenberg, M. E. Eotaxin-3 and a uniquely conserved gene-expression profile in eosinophilic esophagitis. J. Clin. Invest. 116: 536-547, 2006. 2. Takeda, A., Baffi, J. Z., Kleinman, M. E., Cho, W. G., Nozaki, M., Yamada, K., Kaneko, H., Albuquerque, R. J. C., Dridi, S., Saito, K., Raisler, B. J., Budd, S. J., and 15 others. CCR3 is a target for age-related macular degeneration diagnosis and therapy. Nature 460: 225-230, 2009.

• Storage Conditions:

  At -20℃ for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

• Observed Molecular Weight:

  39 kDa

• Fragment:

  Rabbit IgG

• Specificity:

  No cross reactivity with other proteins.

• Applications Notes:

  Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells

• Subcellular Location:

  Cell membrane; Multi-pass membrane protein.