Anti-MCM2 Antibody Picoband®, iFluor647 Conjugate

• Background:

  MCM2 (MINICHROMOSOME MAINTENANCE, S. CEREVISIAE, HOMOLOG OF, 2),also known as MITOTIN, CDCL1 or BM28, is a human nuclear protein that plays an important role in 2 crucial steps of the cell cycle, namely, onset of DNA replication and cell division. And it is similar to members of the family of early S-phase proteins. The MCM2 gene is mapped to 3q21.3. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. In the G0 stage, the MCM2 and MCM5 proteins were much less abundant than the MCM7 and MCM3 proteins, which suggests that the MCM proteins are not present in stoichiometric amounts and that only a proportion of these molecules actively participate in cell cycle regulation as part of MCM complexes.

• Description:

  Boster Bio Anti-MCM2 Antibody catalog # PA1711. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Synonyms:

  DNA replication licensing factor MCM2;3.6.4.12;Minichromosome maintenance protein 2 homolog;Nuclear protein BM28;Mcm2;Bm28, Cdcl1, Kiaa0030, Mcmd2;

• Gene Name:

  DNA replication licensing factor MCM2

• UniProt:

  P97310

• Host:

  Rabbit

• Reactivity:

  Mouse,Rat

• Cross Reactivity:

  No cross-reactivity with other proteins

• Immunogen:

  A synthetic peptide corresponding to a sequence at the C-terminus of mouse MCM2, different from the related rat sequence by one amino acid.

• Clonality:

  Polyclonal

• Applications:

  Flow Cytometry,IF,IHC,ICC,WB

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

• Function:

  Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.

• Components:

  Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

• References & Citations:

  Blow, J. J., Laskey, R. A. A role for the nuclear envelope in controlling DNA replication within the cell cycle. Nature 332: 546-548, 1988. 2. Burkhart, R., Schulte, D., Hu, D., Musahl, C., Gohring, F., Knippers, R. Interactions of human nuclear proteins P1Mcm3 and P1Cdc46. Europ. J. Biochem. 228: 431-438, 1995. 3. Todorov IT, Lavigne J, Sakr F, Kaneva R, Foisy S, Bibor-Hardy V (Jul 1991). "Nuclear matrix protein mitotin messenger RNA is expressed at constant levels during the cell cycle". Biochem Biophys Res Commun 177 (1): 395–400.

• Storage Conditions:

  Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  102078 MW

• Observed Molecular Weight:

  102 kDa

• Fragment:

  Rabbit IgG

• Specificity:

  No cross reactivity with other proteins.

• Applications Notes:

  Western blot, 0.1-0.5μg/ml, Mouse, Rat
  Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, By Heat
  Immunocytochemistry , 0.5-1μg/ml, Mouse, Rat
  Immunocytochemistry/Immunofluorescence, 5μg/ml, Mouse
  Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Mouse
  

• Subcellular Location:

  Nucleus .

• Sequence Similarities:

  Belongs to the MCM family.

• Protein Name:

  DNA replication licensing factor MCM2