Anti-CRMP2/DPYSL2 Antibody Picoband®, iFluor647 Conjugate

• Background:

  Dihydropyrimidinase-related protein 2 is an enzyme that in humans is encoded by the DPYSL2 gene. This gene encodes a member of the collapsin response mediator protein family. Collapsin response mediator proteins form homo- and hetero-tetramers and facilitate neuron guidance, growth and polarity. The encoded protein promotes microtubule assembly and is required for Sema3A-mediated growth cone collapse, and also plays a role in synaptic signaling through interactions with calcium channels. This gene has been implicated in multiple neurological disorders, and hyperphosphorylation of the encoded protein may play a key role in the development of Alzheimer's disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

• Description:

  Boster Bio Anti-CRMP2/DPYSL2 Antibody Picoband® catalog # A02860-4. Tested in IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Synonyms:

  Talin-1; TLN1; KIAA1027; TLN

• Gene Name:

  talin 1

• Gene ID:

  1808

• UniProt:

  Q16555

• Host:

  Rabbit

• Reactivity:

  Human,Mouse,Rat

• Cross Reactivity:

  No cross-reactivity with other proteins.

• Immunogen:

  A synthetic peptide corresponding to a sequence at the N-terminus of human CRMP2/DPYSL2, which shares 94.1% amino acid (aa) sequence identity with both mouse and rat CRMP2/DPYSL2.

• Clonality:

  Polyclonal

• Tissue Specificity:

  Placenta and skin. Predominantly expressed in mammary gland tissue.

• Applications:

  IF,ICC,WB

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Function:

  Probably involved in connections of major cytoskeletal structures to the plasma membrane. High molecular weight cytoskeletal protein concentrated at regions of cell-substratum contact and, in lymphocytes, at cell-cell contacts (By similarity).

• Components:

  Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

• References & Citations:

  1. Abe, H., Jitsuki, S., Nakajima, W., Murata, Y., Jitsuki-Takahashi, A., Katsuno, Y., Tada, H., Sano, A., Suyama, K., Mochizuki, N., Komori, T., Masuyama, H., Okuda, T., Goshima, Y., Higo, N., Takahashi, T. CRMP2-binding compound, edonerpic maleate, accelerates motor function recovery from brain damage. Science 360: 50-57, 2018. 2. Brittain, J. M., Duarte, D. B., Wilson, S. M., Zhu, W., Ballard, C., Johnson, P. L., Liu, N., Xiong, W., Ripsch, M. S., Wang, Y., Fehrenbacher, J. C., Fitz, S. D., and 20 others. Suppression of inflammatory and neuropathic pain by uncoupling CRMP-2 from the presynaptic Ca(2+) channel complex. Nature Med. 17: 822-829, 2011. 3. Fukata, Y., Itoh, T. J., Kimura, T., Menager, C., Nishimura, T., Shiromizu, T., Watanabe, H., Inagaki, N., Iwamatsu, A., Hotani, H., Kaibuchi, K. CRMP-2 binds to tubulin heterodimers to promote microtubule assembly. Nature Cell Biol. 4: 583-591, 2002.

• Storage Conditions:

  At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

• Calculated Molecular Weight:

  36043 MW

• Observed Molecular Weight:

  65 kDa

• Fragment:

  Rabbit IgG

• Applications Notes:

  Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
  Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
  

• Subcellular Location:

  Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytoskeleton. Cell surface.

• Protein Name:

  Melanocortin receptor 3