Anti-PLD2 Picoband® Antibody-iFluor647 Conjugate

• Background:

  Phospholipase D2 is an enzyme that in humans is encoded by the PLD2 gene. It is mapped to 17p13.2. The protein encoded by this gene catalyzes the hydrolysis of phosphatidylcholine to phosphatidic acid and choline. The activity of the encoded enzyme is enhanced by phosphatidylinositol 4,5-bisphosphate and ADP-ribosylation factor-1. This protein localizes to the peripheral membrane and may be involved in cytoskeletal organization, cell cycle control, transcriptional regulation, and/or regulated secretion. Two transcript variants encoding different isoforms have been found for this gene.

• Description:

  Boster Bio Anti-PLD2 Picoband® Antibody catalog # A02586. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Synonyms:

  Phospholipase D2; PLD 2; hPLD2; Choline phosphatase 2; PLD1C; Phosphatidylcholine-hydrolyzing phospholipase D2; PLD2

• Gene Name:

  phospholipase D2

• Gene ID:

  5338

• UniProt:

  O14939

• Host:

  Rabbit

• Reactivity:

  Human,Mouse,Rat

• Cross Reactivity:

  No cross-reactivity with other proteins.

• Immunogen:

  E.coli-derived human PLD2 recombinant protein (Position: A37-E924).

• Clonality:

  Polyclonal

• Tissue Specificity:

  Widely expressed.

• Applications:

  WB,ICC,IF,Flow Cytometry,ELISA

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

• Function:

  May have a role in signal-induced cytoskeletal regulation and/or endocytosis.

• Components:

  Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

• References & Citations:

  1. Liscovitch, M., Czarny, M., Fiucci, G., Tang, X. Phospholipase D: molecular and cell biology of a novel gene family. Biochem. J. 345: 401-415, 2000. 2. Lopez, I., Arnold, R. S., Lambeth, J. D. Cloning and initial characterization of a human phospholipase D2 (hPLD2): ADP-ribosylation factor regulated hPLD2. J. Biol. Chem. 273: 12846-12852, 1998. 3. Park, S. H., Ryu, S. H., Suh, P. G., Kim, H. Assignment of human PLD2 to chromosome band 17p13.1 by fluorescence in situ hybridization. Cytogenet. Cell Genet. 82: 225 only, 1998.

• Storage Conditions:

  Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

• Observed Molecular Weight:

  106 kDa

• Fragment:

  Rabbit IgG

• Specificity:

  No cross reactivity with other proteins.

• Applications Notes:

  Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
  Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
  Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human
  ELISA, 0.1-0.5μg/ml, -
  

• Subcellular Location:

  Membrane. Peripheral membrane protein.