Anti-MCP1/CCL2 Antibody Picoband® (iFluor647 Conjugate)

• Background:

  Monocyte chemoattractant protein-1 (MCP-1), a member of the chemokine (chemotactic cytokine) family, is a potent monocyte agonist that is upregulated by oxidized lipids. MCP-1 is also known as CCL2, SCYA2, MCAF. MCAF is a member of family of factors involved in immune and inflammatory responses. The amino acid sequence deduced from the nucleotide sequence reveals the primary structure of the MCAF precursor to be composed of a putative signal peptide sequence of 23 amino acid residues and a mature MCAF sequence of 76 amino acid residues. MCP-1 plays a unique and crucial role in the initiation of atherosclerosis and may provide a new therapeutic target in this disorder. Human MCP-1 is a 8.7KDa non-glycoprotein, consisting of 99 amino acids in precursor form and 76 amino acids in mature form.

• Description:

  Boster Bio Anti-MCP1/CCL2 Antibody Picoband® catalog # PB9570. Tested in ELISA, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Synonyms:

  C-C motif chemokine 2;HC11;Monocyte chemoattractant protein 1;Monocyte chemotactic and activating factor;MCAF;Monocyte chemotactic protein 1;MCP-1;Monocyte secretory protein JE;Small-inducible cytokine A2;CCL2;MCP1, SCYA2;

• Gene Name:

  C-C motif chemokine 2

• UniProt:

  P13500

• Host:

  Rabbit

• Reactivity:

  Human

• Cross Reactivity:

  No cross-reactivity with other proteins

• Immunogen:

  E. coli-derived human MCP-1 recombinant protein (Position: Q24-T99). Human MCP-1 shares 60.9% and 59.4% amino acid (aa) sequence identity with mouse and rat MCP-1, respectively.

• Clonality:

  Polyclonal

• Tissue Specificity:

  Synthesized in liver and selectively concentrated in bone matrix. Secreted in plasma. It is also found in dentin in much higher quantities than other plasma proteins.

• Applications:

  ELISA,IHC,WB

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

• Function:

  Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.

• Storage Conditions:

  Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

• Fragment:

  Rabbit IgG

• Applications Notes:

  Western blot, 0.1-0.5μg/ml, Human
  Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
  ELISA, 0.1-0.5μg/ml, -
  

• Subcellular Location:

  Secreted.

• Sequence Similarities:

  Belongs to the intercrine beta (chemokine CC) family.

• Protein Name:

  C-C motif chemokine 2