Anti-Myeloperoxidase/MPO Antibody Picoband®, APC Conjugate

• Background:

  Myeloperoxidase (MPO) is a mammalian phagocyte hemoprotein thought to primarily mediate host defense reactions. It is abundantly expressed in neutrophils and secreted during their activation. Myeloperoxidase is part of the host defense system of human polymorphonuclear leukocytes, responsible for microbicidal activity against a wide range of organisms. It is located in the nucleus as well as in the cytoplasm. Intranuclear MPO may help to protect DNA against damage resulting from oxygen radicals produced during myeloid cell maturation and function. The standard product used in this kit is the product of gene recombination, consisting of 697 (A49-S745) amino acids with the molecular mass of 80KDa.

• Description:

  Boster Bio Anti-Myeloperoxidase/MPO Antibody Picoband® catalog # PB9057. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Synonyms:

  Myeloperoxidase;MPO;1.11.2.2;Myeloperoxidase;89 kDa myeloperoxidase;84 kDa myeloperoxidase;Myeloperoxidase light chain;Myeloperoxidase heavy chain;MPO;

• Gene Name:

  Myeloperoxidase

• Gene ID:

  4353

• UniProt:

  P05164

• Host:

  Rabbit

• Reactivity:

  Human

• Cross Reactivity:

  No cross-reactivity with other proteins

• Immunogen:

  E.coli-derived human Myeloperoxidase recombinant protein (Position:S406-S745). Human Myeloperoxidase shares 90% amino acid (aa) sequence identity with mouse Myeloperoxidase.

• Clonality:

  Polyclonal

• Tissue Specificity:

  Mast cells in lung, heart, skin and placenta. Expressed in both normal skin and in urticaria pigmentosa lesions. .

• Applications:

  Flow Cytometry,IHC,WB

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

• Function:

  Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.

• Components:

  Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

• References & Citations:

  1. Klebanoff, S. J. : Myeloperoxidase. Proc. Assoc. Am. Phys. 111: 383-389, 1999. 2. Murao, S.-I.; Stevens, F. J.; Ito, A.; Huberman, E. : Myeloperoxidase: a myeloid cell nuclear antigen with DNA-binding properties. Proc. Nat. Acad. Sci. 85: 1232-1236, 1988. 3. Nauseef, W. M.; Olsson, I.; Arnljots, K. : Biosynthesis and processing of myeloperoxidase--a marker for myeloid cell differentiation. Europ. J. Haemat. 40: 97-110, 1988.

• Storage Conditions:

  Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  83869 MW

• Observed Molecular Weight:

  60-65 kDa, 80-90 kDa

• Fragment:

  Rabbit IgG

• Specificity:

  No cross reactivity with other proteins.

• Applications Notes:

  Western blot, 0.1-0.5μg/ml, Human
  Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
  Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
  

• Subcellular Location:

  Lysosome.

• Sequence Similarities:

  Belongs to the peroxidase family. XPO subfamily.

• Protein Name:

  Myeloperoxidase