Anti-Calponin/CNN1 Antibody Picoband®, APC Conjugate

Anti-Calponin/CNN1 Antibody Picoband®, APC Conjugate

• Background: Calponin is a basic 34-kD protein first isolated from chicken gizzard and bovine aorta. This gene is mapped to 19p13.2. It is specifically expressed in smooth muscle and binds calmodulin, actin, and tropomyosin. It is able to inhibit the ATPase activity of myosin and is though to play a role in smooth muscle contraction. Calponin is a smooth muscle specific, actin-, tropomyosin- and calmodulin-binding protein though to be involved in some way in the regulation or modulation of contraction.
• Description: Boster Bio Anti-Calponin/CNN1 Antibody catalog # PA2224. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
• Synonyms: Calponin-1;Basic calponin;Calponin H1, smooth muscle;CNN1;
• Gene Name: Calponin-1
• UniProt: P51911
• Host: Rabbit
• Reactivity: Human,Mouse,Rat
• Cross Reactivity: No cross-reactivity with other proteins
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Calponin, different from the related mouse and rat sequences by two amino acids.
• Clonality: Polyclonal
• Tissue Specificity: Smooth muscle, and tissues containing significant amounts of smooth muscle.
• Applications: IHC,WB
• Purification: Immunogen affinity purified.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form: Lyophilized
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Function: Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity (By similarity). .
• Components: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• References & Citations: 1. Miano, J. M., Krahe, R., Garcia, E., Elliott, J. M., Olson, E. N. Expression, genomic structure and high resolution mapping to 19p13.2 of the human smooth muscle cell calponin gene. Gene 197: 215-224, 1997. 2. Miano, J. M., Thomas, S., Disteche, C. M. Expression and chromosomal mapping of the mouse smooth muscle calponin gene. Mammalian Genome 12: 187-191, 2001. 3. Strasser, P., Gimona, M., Moessler, H., Herzog, M., Small, J. V. Mammalian calponin: identification and expression of genetic variants. FEBS Lett. 330: 13-18, 1993.
• Storage Conditions: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
• Calculated Molecular Weight: 33170 MW
• Observed Molecular Weight: 33 kDa
• Fragment: Rabbit IgG
• Specificity: No cross reactivity with other proteins.
• Applications Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
  Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
  
• Subcellular Location: Nucleus . Cytoplasm . Accumulates in aggresomes under proteasome inhibition conditions.
• Sequence Similarities: Belongs to the calponin family.
• Protein Name: Calponin-1