Anti-SSR3 Antibody Picoband®, APC Conjugate

• Background:

  SSR3 (signal sequence receptor, gamma) is also known as TRAPG. By immunoaffinity chromatography, biochemical purification, peptide sequence analysis, and screening of a rat liver cDNA library, Hartmann et al. (1993) obtained a cDNA encoding Ssr3, which they termed Trap-gamma. The deduced 185-amino acid protein spans the membrane 4 times and is expressed as a 20-kD protein. Gross (2012)mapped the SSR3 gene to chromosome 3q25.31 based on an alignment of the SSR3 sequence with the genomic sequence (GRCh37). Hartmann et al. (1993)found that Ssr3 was associated in equal amounts with Ssr1, Ssr2, and Ssr4 in canine pancreatic microsomes.

• Description:

  Boster Bio Anti-SSR3 Antibody catalog # PA1796. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Synonyms:

  Translocon-associated protein subunit gamma;TRAP-gamma;Signal sequence receptor subunit gamma;SSR-gamma;SSR3;TRAPG;

• Gene Name:

  Translocon-associated protein subunit gamma

• UniProt:

  Q9UNL2

• Host:

  Rabbit

• Reactivity:

  Human,Monkey,Mouse,Rat

• Cross Reactivity:

  No cross-reactivity with other proteins

• Immunogen:

  A synthetic peptide corresponding to a sequence at the N-terminus of human SSR3, identical to the related rat and mouse sequences.

• Clonality:

  Polyclonal

• Tissue Specificity:

  Highly expressed in the brain, lung, kidney and heart. Expressed at low levels in the liver and spleen. .

• Applications:

  IF,IHC,ICC,WB

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

• Function:

  TRAP proteins are part of a complex whose function is to bind calcium to the ER membrane and thereby regulate the retention of ER resident proteins.

• Components:

  Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

• References & Citations:

  1. Gross, M. B.Personal Communication.Baltimore, Md. 6/20/2012. 2. Hartmann, E., Gorlich, D., Kostka, S., Otto, A., Kraft, R., Knespel, S., Burger, E., Rapoport, T. A., Prehn, S.A tetrameric complex of membrane proteins in the endoplasmic reticulum.Europ. J. Biochem. 214: 375-381, 1993.

• Storage Conditions:

  Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  21080 MW

• Observed Molecular Weight:

  21 kDa

• Fragment:

  Rabbit IgG

• Specificity:

  No cross reactivity with other proteins.

• Applications Notes:

  Western blot, 0.1-0.5μg/ml, Human, Monkey, Mouse, Rat
  Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Mouse, Rat, By Heat
  Immunocytochemistry , 0.5-1μg/ml, Human
  Immunofluorescence, 5 μg/ml, Human, Mouse, Rat
  

• Subcellular Location:

  Endoplasmic reticulum membrane; Multi-pass membrane protein.

• Protein Name:

  Translocon-associated protein subunit gamma(TRAP-gamma)