Anti-NIP7 Antibody Picoband®, APC Conjugate

• Background:

  60S ribosome subunit biogenesis protein NIP7 homolog is a protein that in humans is encoded by the NIP7 gene. NIP7 is required for proper 27S pre-rRNA processing and 60S ribosome subunit assembly.

• Description:

  Boster Bio Anti-NIP7 Antibody Picoband® catalog # A08120-2. Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Gene Name:

  NIP7

• Gene ID:

  51388

• UniProt:

  Q9Y221

• Host:

  Rabbit

• Reactivity:

  Human,Mouse

• Immunogen:

  E.coli-derived human NIP7 recombinant protein (Position: E14-T180). Human NIP7 shares 97.6% and 97% amino acid (aa) sequence identity with mouse and rat NIP7, respectively.

• Clonality:

  Polyclonal

• Applications:

  WB,Flow Cytometry,ELISA

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Components:

  Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

• References & Citations:

  1. Gross, M. B. Personal Communication. Baltimore, Md. 2/23/2021. 2. Liu, J.-F., Wang, X.-Q., Wang, Z.-X., Chen, J.-R., Jiang, T., An, X.-M., Chang, W.-R., Liang, D.-C. Crystal structure of KD93, a novel protein expressed in human hematopoietic stem/progenitor cells. J. Struct. Biol. 148: 370-374, 2004. 3. Morello, L. G., Coltri, P. P., Quaresma, A. J. C., Simabuco, F. M., Silva, T. C. L., Singh, G. Nickerson, J. A., Oliveira, C. C., Moore, M. J., Zanchin, N. I. T. The human nucleolar protein FTSJ3 associates with NIP7 and functions in pre-rRNA processing. PLoS One 6: e29174, 2011. Note: Electronic Article.

• Storage Conditions:

  At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

• Observed Molecular Weight:

  20 kDa

• Applications Notes:

  Western blot, 0.25-0.5 μg/ml, Human, Mouse
  Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
  ELISA, 0.1-0.5 μg/ml, -