Anti-Mkl1/MRTFA Antibody Picoband®, APC Conjugate

• Background:

  The protein encoded by this gene interacts with the transcription factor myocardin, a key regulator of smooth muscle cell differentiation. The encoded protein is predominantly nuclear and may help transduce signals from the cytoskeleton to the nucleus. This gene is involved in a specific translocation event that creates a fusion of this gene and the RNA-binding motif protein-15 gene. This translocation has been associated with acute megakaryocytic leukemia. Alternative splicing results in multiple transcript variants.

• Description:

  Boster Bio Anti-Mkl1/MRTFA Antibody Picoband® catalog # A32468-2. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Synonyms:

  Protein Bop; BH3-only protein; Retrotransposon Gag-like protein 10; RTL10; BOP, C22orf29

• Gene Name:

  retrotransposon Gag like 10

• Gene ID:

  57591

• UniProt:

  Q969V6

• Host:

  Rabbit

• Reactivity:

  Human

• Cross Reactivity:

  No cross-reactivity with other proteins.

• Immunogen:

  E.coli-derived human Mkl1/MRTFA recombinant protein (Position: L192-L931).

• Clonality:

  Polyclonal

• Tissue Specificity:

  Ubiquitously expressed.

• Applications:

  ELISA,IF,ICC,WB

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Function:

  Could induce apoptosis in a BH3 domain-dependent manner. The interaction network of Bcl-2 family members may play a key role in modulation RTL10/BOP intrinsic apoptotic signaling activity.

• Components:

  Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

• References & Citations:

  1. Brandt, D. T., Baarlink, C., Kitzing, T. M., Kremmer, E., Ivaska, J., Nollau, P., Grosse, R. SCAI acts as a suppressor of cancer cell invasion through the transcriptional control of beta-1-integrin. Nature Cell Biol. 11: 557-567, 2009. 2. Cen, B., Selvaraj, A., Burgess, R. C., Hitzler, J. K., Ma, Z., Morris, S. W., Prywes, R. Megakaryoblastic leukemia 1, a potent transcriptional coactivator for serum response factor (SRF), is required for serum induction of SRF target genes. Molec. Cell. Biol. 23: 6597-6608, 2003. 3. Ho, C. Y., Jaalouk, D. E., Vartiainen, M. K., Lammerding, J. Lamin A/C and emerin regulate MKL1-SRF activity by modulating actin dynamics. Nature 497: 507-511, 2013.

• Storage Conditions:

  At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

• Calculated Molecular Weight:

  14377 MW

• Observed Molecular Weight:

  145 kDa

• Fragment:

  Rabbit IgG

• Applications Notes:

  Western blot, 0.25-0.5 μg/ml, Human
  Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
  ELISA, 0.1-0.5 μg/ml, -
  

• Subcellular Location:

  Cell membrane; Lipid-anchor, GPI-anchor.

• Protein Name:

  Lymphocyte antigen 6A-2/6E-1