Anti-RPL23 Antibody Picoband®, APC Conjugate

• Background:

  60S ribosomal protein L23 is a protein that in humans is encoded by the RPL23 gene. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L14P family of ribosomal proteins. It is located in the cytoplasm. This gene has been referred to as rpL17 because the encoded protein shares amino acid identity with ribosomal protein L17 from Saccharomyces cerevisiae; however, its official symbol is RPL23. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

• Description:

  Boster Bio Anti-RPL23 Antibody Picoband® catalog # A06155-2. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Synonyms:

  S-adenosylmethionine decarboxylase proenzyme; AdoMetDC; SAMDC; 4.1.1.50

• Gene Name:

  adenosylmethionine decarboxylase 1

• Gene ID:

  9349

• UniProt:

  P62829

• Host:

  Rabbit

• Reactivity:

  Human,Mouse,Rat

• Cross Reactivity:

  No cross-reactivity with other proteins.

• Immunogen:

  E.coli-derived human RPL23 recombinant protein (Position: A12-A136).

• Clonality:

  Polyclonal

• Tissue Specificity:

  Widely expressed with higher expression in lung, skeletal muscle, brain, uterus, ovary, thyroid and prostate.

• Applications:

  WB,IHC,Flow Cytometry,ELISA

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Function:

  Essential for biosynthesis of the polyamines spermidine and spermine. Promotes maintenance and self-renewal of embryonic stem cells, by maintaining spermine levels.

• Components:

  Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

• References & Citations:

  1. Berchtold, M. W., Berger, M. C. Isolation and analysis of a human cDNA highly homologous to the yeast gene encoding L17A ribosomal protein. Gene 102: 283-288, 1991. 2. Dai, M.-S., Shi, D., Jin, Y., Sun, X.-X., Zhang, Y., Grossman, S. R., Lu, H. Regulation of the MDM2-p53 pathway by ribosomal protein L11 involves a post-ubiquitination mechanism. J. Biol. Chem. 281: 24304-24313, 2006. 3. Herault, Y., Michel, D., Chatelain, G., Brun, G. cDNA and predicted amino acid sequences of the human ribosomal protein genes rpS12 and rpL17. Nucleic Acids Res. 19: 4001 only, 1991.

• Storage Conditions:

  At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

• Observed Molecular Weight:

  17 kDa

• Fragment:

  Rabbit IgG

• Applications Notes:

  Western blot, 0.25-0.5 μg/ml, Human, Rat
  Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat
  Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human, Rat
  ELISA, 0.1-0.5 μg/ml, -
  

• Subcellular Location:

  Nucleus.