Anti-RGS9 Antibody Picoband®, FITC Conjugate

• Background:

  Regulator of G-protein signaling 9, also known as RGS9 is a human gene, which codes for a protein involved in regulation of signal transduction inside cells. This gene is mapped to 17q24.1. This gene encodes a member of the RGS family of GTPase activating proteins that function in various signaling pathways by accelerating the deactivation of G proteins. This protein is anchored to photoreceptor membranes in retinal cells and deactivates G proteins in the rod and cone phototransduction cascades. Mutations in this gene result in bradyopsia. Multiple transcript variants encoding different isoforms have been found for this gene.

• Description:

  Boster Bio Anti-RGS9 Antibody catalog # PA2241. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Synonyms:

  Regulator of G-protein signaling 9;RGS9;RGS9;

• Gene Name:

  Regulator of G-protein signaling 9

• UniProt:

  O75916

• Host:

  Rabbit

• Reactivity:

  Human,Mouse,Rat

• Cross Reactivity:

  No cross-reactivity with other proteins

• Immunogen:

  A synthetic peptide corresponding to a sequence at the C-terminus of human RGS9, different from the related mouse and rat sequences by three amino acids.

• Clonality:

  Polyclonal

• Tissue Specificity:

  Highly expressed in the caudate and putamen, lower levels found in the hypothalamus and nucleus accumbens and very low levels in cerebellum. Not expressed in globus pallidus or cingulate cortex. Isoform 2 is expressed predominantly in pineal gland and retina. Isoform 3 is expressed in retina (abundant in photoreceptors).

• Applications:

  WB

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

• Function:

  Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds to G (t)-alpha. Involved in phototransduction; key element in the recovery phase of visual transduction (By similarity). .

• Components:

  Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

• References & Citations:

  1. Cabrera-Vera, T. M., Hernandez, S., Earls, L. R., Medkova, M., Sundgren-Andersson, A. K., Surmeier, D. J., Hamm, H. E. RGS9-2 modulates D2 dopamine receptor-mediated Ca(2+) channel inhibition in rat striatal cholinergic interneurons. Proc. Nat. Acad. Sci. 101: 16339-16344, 2004. 2. Hu, G., Zhang, Z., Wensel, T. G. Activation of RGS9-1GTPase acceleration by its membrane anchor, R9AP. J. Biol. Chem. 278: 14550-14554, 2003. 3. Sierra, D. A., Gilbert, D. J., Householder, D., Grishin, N. V., Yu, K., Ukidwe, P., Barker, S. A., He, W., Wensel, T. G., Otero, G., Brown, G., Copeland, N. G., Jenkins, N. A., Wilkie, T. M. Evolution of the regulators of G-protein signaling multigene family in mouse and human. Genomics 79: 177-185, 2002.

• Storage Conditions:

  Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  76966 MW

• Observed Molecular Weight:

  56 kDa, 77 kDa

• Fragment:

  Rabbit IgG

• Specificity:

  No cross reactivity with other proteins.

• Applications Notes:

  Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
  

• Subcellular Location:

  Isoform 3: Membrane; Peripheral membrane protein. Isoform 3 is targeted to the membrane via its interaction with RGS9BP. .

• Sequence Similarities:

  Contains 1 DEP domain.

• Protein Name:

  Regulator of G-protein signaling 9