Anti-ADAM17 Antibody Picoband®, FITC Conjugate

• Background:

  ADAM17 (ADAM metallopeptidase domain 17), also called TACE  (tumor necrosis factor-alpha-converting enzyme), is a 70-kDa enzyme that belongs to the ADAM protein family of disintegrins and metalloproteases. Expression studies showed that the encoded protein cleaves precursor tumor necrosis factor-alpha to its mature form. Northern blot analysis revealed that the gene was expressed as a 5-kb mRNA in all tissues examined. ADAM17 is understood to be involved in the processing of tumor necrosis factor alpha (TNF-alpha) at the surface of the cell, and from within theintracellular membranes of the trans-Golgi network. This process, which is also known as "shedding", involves the cleavage and release of a soluble ectodomain from membrane-bound pro-proteins (such as pro-TNF-alpha), and is of known physiological importance. ADAM17 was the first "sheddase" to be identified, and is also understood to play a role in the release of a diverse variety of membrane-anchored cytokines, cell adhesion molecules,receptors, ligands, and enzymes.

• Description:

  Boster Bio Anti-ADAM17 Antibody catalog # PA1844. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Synonyms:

  Disintegrin and metalloproteinase domain-containing protein 17;ADAM 17;3.4.24.86;Snake venom-like protease;TNF-alpha convertase;TNF-alpha-converting enzyme;CD156b;ADAM17;CSVP, TACE;

• Gene Name:

  Disintegrin and metalloproteinase domain-containing protein 17

• UniProt:

  P78536

• Host:

  Rabbit

• Reactivity:

  Rat,Mouse,Human

• Cross Reactivity:

  No cross-reactivity with other proteins

• Immunogen:

  A synthetic peptide corresponding to a sequence at the C-terminus of human ADAM17, different from the related rat and mouse sequences by one amino acid.

• Clonality:

  Polyclonal

• Tissue Specificity:

  Ubiquitously expressed. Expressed at highest levels in adult heart, placenta, skeletal muscle, pancreas, spleen, thymus, prostate, testes, ovary and small intestine, and in fetal brain, lung, liver and kidney.

• Applications:

  WB

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

• Function:

  Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Acts as an activator of Notch pathway by mediating cleavage of Notch, generating the membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). Plays a role in the proteolytic processing of ACE2. .

• Components:

  Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

• References & Citations:

  1. Black, R. A., Rauch, C. T., Kozlosky, C. J., Peschon, J. J., Slack, J. L., Wolfson, M. F., Castner, B. J., Stocking, K. L., Reddy, P., Srinivasan, S., Nelson, N., Boiani, N., Schooley, K. A., Gerhart, M., Davis, R., Fitzner, J. N., Johnson, R. S., Paxton, R. J., March, C. J., Cerretti, D. P. A metalloproteinase disintegrin that releases tumour-necrosis factor-alpha from cells. Nature 385: 729-733, 1997.

• Storage Conditions:

  Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  93021 MW

• Observed Molecular Weight:

  95 kDa

• Fragment:

  Rabbit IgG

• Specificity:

  No cross reactivity with other proteins.

• Applications Notes:

  Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
  

• Subcellular Location:

  Membrane; Single-pass type I membrane protein.

• Sequence Similarities:

  Contains 1 disintegrin domain.

• Protein Name:

  Disintegrin and metalloproteinase domain-containing protein 17(ADAM 17)