Anti-SLD5/GINS4 Antibody Picoband®, FITC Conjugate

• Background:

  The yeast heterotetrameric GINS complex is made up of Sld5, Psf1 (GINS1; MIM 610608), Psf2 (GINS2; MIM 610609), and Psf3 (GINS3; MIM 610610). The formation of the GINS complex is essential for the initiation of DNA replication in yeast and Xenopus egg extracts (Ueno et al., 2005 [PubMed 16287864]). See GINS1 for additional information about the GINS complex.

• Description:

  Boster Bio Anti-SLD5/GINS4 Antibody Picoband® catalog # A10548-1. Tested in WB, IHC, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Gene Name:

  GINS4

• Gene ID:

  84296

• UniProt:

  Q9BRT9

• Host:

  Rabbit

• Reactivity:

  Human,Mouse,Rat

• Immunogen:

  E.coli-derived human SLD5/GINS4 recombinant protein (Position: M1-I223). Human GINS4 shares 87.9% and 88.8% amino acid (aa) sequence identity with mouse and rat GINS4, respectively.

• Clonality:

  Polyclonal

• Applications:

  WB,IHC,Flow Cytometry,ELISA

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Components:

  Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

• References & Citations:

  1. Kong, L., Ueno, M., Itoh, M., Yoshioka, K., Takakura, N. Identification and characterization of mouse PSF1-binding protein, SLD5. Biochem. Biophys. Res. Commun. 339: 1204-1207, 2006. 2. Takayama, Y., Kamimura, Y., Okawa, M., Muramatsu, S., Sugino, A., Araki, H. GINS, a novel multiprotein complex required for chromosomal DNA replication in budding yeast. Genes Dev. 17: 1153-1165, 2003. 3. Ueno, M., Itoh, M., Kong, L., Sugihara, K., Asano, M., Takakura, N. PSF1 is essential for early embryogenesis in mice. Molec. Cell Biol. 25: 10528-10532, 2005.

• Storage Conditions:

  At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

• Calculated Molecular Weight:

  26 kDa

• Observed Molecular Weight:

  28 kDa

• Applications Notes:

  Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
  Immunohistochemistry, 2-5 μg/ml, Human
  Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
  ELISA, 0.1-0.5 μg/ml, -